甘蓝β-淀粉酶基因家族的鉴定及其响应低温胁迫的表达分析  

Identification ofβ-amylase gene family in cabbage and the expression analysis in response to low temperature stress

作　　者：山溪 陶美奇 潘永飞[1] 秦文斌[1] 张振超[1] 姚悦梅[1] 戴忠良[1] SHAN Xi;TAO Meiqi;PAN Yongfei;QIN Wenbin;ZHANG Zhenchao;YAO Yuemei;DAI Zhongliang(Zhenjiang Institute of Agricultural Sciences of the Ning-Zhen Hilly District,Jurong 212400,China)

机构地区：[1]江苏丘陵地区镇江农业科学研究所,江苏句容212400

出　　处：《江苏农业学报》2025年第2期345-354,共10页Jiangsu Journal of Agricultural Sciences

基　　金：2024年度江苏省青年科技人才托举工程项目(JSTJ-2024-610);江苏省重点研发计划项目(BE2021399)。

摘　　要：β-淀粉酶(BAM)是植物水解淀粉的重要酶类,在植物应对非生物胁迫响应中有重要作用。本研究基于甘蓝BRAD参考基因组的全基因组序列,分离鉴定得到20个BolBAM基因,并对其家族成员进行系统发育分析、蛋白质特征分析、基因结构分析、不同器官/组织中的相对表达量分析、低温(2℃)胁迫下的基因表达模式分析。结果表明,从甘蓝中共鉴定得到20个BolBAM蛋白,BolBAM蛋白的氨基酸序列长度范围是193 aa(BolBAM3c)~678 aa(BolBAM7);甘蓝BAM蛋白家族成员兼有弱酸性、弱碱性蛋白质;除BolBAM11、BolBAM12、BolBAM14外,其他BolBAM蛋白均为亲水性蛋白质。RNA-Seq(转录组)分析结果表明,BolBAM1b、BolBAM5a、BolBAM9b在7个组织/器官中的相对表达量均较高,在花中BolBAM1b的相对表达量最高,在角果中BolBAM5a的相对表达量最高,在愈伤组织中BolBAM9b的相对表达量最高。本研究还发现,在甘蓝耐冷材料923、冷敏材料D9中,14个BolBAM基因受到低温诱导表达;冷敏材料D9中BolBAM3a的相对表达量在低温处理6 h时高于耐冷材料923,但在低温处理24 h时,其相对表达量显著低于923;耐冷材料923中BolBAM3b的相对表达量在低温处理6 h、24 h时均显著高于冷敏材料D9。本研究结果为后续开展BolBAM3a、BolBAM3b基因调控甘蓝应对低温胁迫的研究提供了重要参考。β-amylase(BAM)is an important enzyme in the hydrolysis of plant starch,playing a crucial role in the response of plants to abiotic stress.This study,which was based on the whole genome sequence of the cabbage BRAD reference genome,identified 20 BolBAM genes.The phylogenetic analysis,protein characteristics,gene structure,and expression levels in different organs or tissues of its family members were analyzed.Additionally,the gene expression patterns under 2℃low temperature stress were also examined.The results showed that a total of 20 BolBAM proteins were identified in cabbage.The amino acid sequence length of BolBAM proteins ranged from 193 aa(BolBAM3c)to 678 aa(BolBAM7).Members of the cabbage BAM protein family included both weakly acidic and weakly alkaline proteins.Except for BolBAM11,BolBAM12,and BolBAM14,all other BolBAM proteins were hydrophilic.The RNA-Seq results showed that BolBAM1b,BolBAM5a,and BolBAM9b had relatively high expression levels in seven tissues or organs,with BolBAM1b showing the highest expression in flowers,BolBAM5a in pods,and BolBAM9b in callus tissue.This study found that the expression of 14 BolBAM genes in the cold-resistant material 923 and cold-sensitive material D9 of cabbage was induced by low temperature.The relative expression of BolBAM3a in cold-sensitive material D9 was higher than that in cold-resistant material 923 at 6 h of low temperature treatment,but it was significantly lower than that in cold-resistant material 923 at 24 h of low temperature treatment.The relative expression of BolBAM3b in cold-resistant material 923 was significantly higher than that in cold-sensitive material D9 at 6 h and 24 h of low temperature treatment.This study provides important insights for further research on the regulation of low temperature response in cabbage by the BolBAM3a and BolBAM3b genes.

关 键 词：甘蓝 BAM基因家族 低温胁迫 亚细胞定位 

分 类 号：S635.01[农业科学—蔬菜学]