油菜素内酯浸种对盐胁迫下曼陀罗种子萌发的影响及其生理机制  

The effect of soaking seeds with brassinolide on the germination of Datura stramonium L.seeds under salt stress and its physiological mechanism

作　　者：闫艳华[1] 曾慧琳 杨阳[1] YAN Yanhua;ZENG Huilin;YANG Yang(Department of Biological and Food Engineering,Lyuliang University,Lüliang 033000,China)

机构地区：[1]吕梁学院生物与食品工程系,山西吕梁033000

出　　处：《江苏农业学报》2025年第2期355-361,共7页Jiangsu Journal of Agricultural Sciences

基　　金：吕梁市科技局社会发展领域重点项目(2023SHFZ33);山西省高等学校教学改革创新项目(J20221138)。

摘　　要：在全世界范围内,土壤盐渍化日渐严重,严重威胁植物的生长。为探究油菜素内酯在曼陀罗应对盐胁迫中的作用,本研究用油菜素内酯浸泡曼陀罗种子,分析其对曼陀罗种子萌发、种苗生长、抗氧化酶活性的影响。研究结果表明,NaCl胁迫会抑制曼陀罗种子萌发和种苗生长。1×10^(-9) mol/L油菜素内酯浸种处理可显著提高盐胁迫下曼陀罗种苗的根长、下胚轴长、含水率、发芽率、发芽指数、发芽势、种子活力指数,降低曼陀罗种苗中丙二醛和脯氨酸的含量,提升过氧化物酶(POD)和超氧化物歧化酶(SOD)的活性,增强抗盐胁迫能力。1×10^(-8) mol/L油菜素内酯浸种处理会抑制曼陀罗种子的萌发及种苗生长。本研究为增强曼陀罗抗盐胁迫能力提供理论依据。Worldwide,soil salinization is becoming increasingly severe,posing a serious threat to plant growth.To explore the role of brassinolide in Datura stramonium’s response to salt stress,we soaked the seeds of Datura stramonium with brassinolide and analyzed its effects on seed germination,seedling growth,and antioxidant enzyme activity.The results showed that NaCl stress inhibited the germination of Datura stramonium seeds and the growth of seedlings.Seed soaking treatment with 1×10^(-9) mol/L brassinolide significantly improved the root length,hypocotyl length,water content,germination rate,germination index,germination vigor,and seed vigor index of Datura stramonium under salt stress.It also reduced the content of malondialdehyde and proline in Datura stramonium seedlings,increased the activity of peroxidase(POD)and superoxide dismutase(SOD),and enhanced the resistance to salt stress.Seed soaking treatment with 1×10^(-8) mol/L brassinolide inhibited the germination of Datura stramonium seeds and the growth of seedlings.This study provides a theoretical basis for enhancing the salt stress resistance of Datura stramonium.

关 键 词：油菜素内酯 盐胁迫 曼陀罗 种子萌发 抗氧化酶活性 

分 类 号：Q946.885[生物学—植物学]