堇叶碎米荠富硒多肽对乙醇诱导小鼠肝脏氧化应激损伤的保护作用  

Protective Effect of Selenium-Enriched Peptide of Cardamine violifolia on Alcohol-Induced Oxidative Stress Damage in the Liver of Mice

作　　者：李一晴 丛欣 李玥[3] 陈尚卫 朱松 LI Yiqing;CONG Xin;LI Yue;CHEN Shangwei;ZHU Song(State Key Laboratory of Food Science and Resources,Jiangnan University,Wuxi 214122,China;Enshi Deyuan Selenium Material Engineering Technology Co.,Ltd.,Enshi 445000,China;School of Food Science and Technology,Jiangnan University,Wuxi 214122,China)

机构地区：[1]江南大学食品科学与资源挖掘全国重点实验室,江苏无锡214122 [2]恩施德源硒材料工程科技有限公司,湖北恩施445000 [3]江南大学食品学院,江苏无锡214122

出　　处：《食品与生物技术学报》2024年第12期81-91,共11页Journal of Food Science and Biotechnology

基　　金：国家自然科学基金项目(32172197)。

摘　　要：【目的】探究堇叶碎米荠富硒多肽(selenium-enriched peptide of Cardamine violifolia,SPE)对乙醇诱导小鼠肝脏氧化应激损伤的保护作用。【方法】通过梯度乙醇干预建立了酒精性肝损伤动物模型,以SPE干预模型动物,考察其对小鼠血清生化指标、肝脏氧化应激指标、肝脏组织病理学和肝脏代谢谱的影响。【结果】SPE可以显著缓解乙醇诱导的肝脏损伤,提高肝脏中抗氧化酶的水平。代谢组学的结果表明,SPE显著改善了小鼠肝脏中37种代谢物的表达水平,涉及不饱和脂肪酸的生物合成、亚油酸代谢和甘油磷脂代谢等代谢途径。【结论】SPE对乙醇诱导的小鼠肝脏氧化应激损伤具有保护作用,其机制可能与亚油酸代谢激活、不饱和脂肪酸的生物合成和甘油磷脂代谢有关。[Objective]This study aimed to investigate the protective effect of selenium-enriched peptide of Cardamine violifolia(SPE)on the alcohol-induced oxidative stress damage in the liver of mice.[Method]A mouse model of alcoholic liver damage was established by gradient alcohol gavage.The effects of SPE on the serum levels of biochemical indicators,liver oxidative stress indicators,liver histopathology and liver metabolic profile in mice were studied.[Result]SPE significantly alleviated alcohol-induced liver damage and elevated the levels of antioxidant enzymes in the liver.Metabolomics results showed that SPE significantly recovered the levels of 37 metabolites in the liver of mice.These metabolites were involved in metabolic pathways such as biosynthesis of unsaturated fatty acids,linoleic acid metabolism,and glycerophospholipid metabolism.[Conclusion]SPE exerts a positive protective effect on alcohol-induced oxidative stress injury in mouse liver by regulating the activation of linoleic acid metabolism,biosynthesis of unsaturated fatty acids,and glycerophospholipid metabolism.

关 键 词：堇叶碎米荠 富硒多肽 乙醇 氧化应激 代谢组学 

分 类 号：R28[医药卫生—中药学]