EPB41通过介导脂肪酸氧化影响食管癌增殖和转移的机制研究  

Effect of EPB41 Mediated Fatty Acid Oxidation on Esophageal Cancer Cell Proliferation and Migration

作　　者：林近秋 王亮[2] 刘石柱 LIN Jinqiu;WANG Liang;LIU Shizhu(Department of Gastroenterology,Xingtai General Hospital,North China Medical and Health Group,Xingtai,Hebei,050041,China;Department of Gastroenterology,Cangzhou Central Hospital,Xingtai,Hebei,061017,China;Department of Gastroenterology,the Second Affiliated Hospital of Xingtai Medical College,Xingtai,Hebei,054002,China)

机构地区：[1]华北医疗健康集团邢台总医院内五科,河北省邢台市054000 [2]沧州市中心医院消化内科,河北省沧州市061000 [3]邢台医学高等专科学校第二附属医院消化内科,河北省邢台市054000

出　　处：《医学分子生物学杂志》2025年第2期153-159,共7页Journal of Medical Molecular Biology

基　　金：2022年河北省医学科学计划研究课题(No.20220386)。

摘　　要：目的探讨蛋白质EPB41(erythrocyte membrane protein band 4.1)对食管癌细胞恶性表型的影响,并基于Wnt/β-catenin信号通路介导的脂肪酸氧化(fatty acid oxidation,FAO)探讨可能的潜在机制。方法将对数生长期的KYSE30细胞及KYSE150细胞各分为5组,检测mRNA和蛋白质表达、细胞增殖和迁移率、ATP水平、NADPH/NADP^(+)比率、活性氧(ROS)和GSH水平。结果在KYSE30细胞中,转染EPB41 siRNA组细胞克隆数、细胞迁移率、ATP水平、NADPH/NADP^(+)比率、ROS水平、细胞中Wnt3a、β-catenin和核β-catenin蛋白质表达均明显增加(P<0.05),且脂肪酸氧化抑制剂和Wnt通路抑制剂可逆转EPB41的作用;在KYSE150细胞中,转染EPB41过表达载体oe-EPB41组细胞克隆数、细胞迁移率、ATP水平、NADPH/NADP^(+)比率、ROS水平、细胞中Wnt3a、β-catenin和核β-catenin蛋白质表达均明显降低(P<0.05),且脂肪酸氧化诱导剂和Wnt通路激活剂可逆转EPB41的作用。结论EPB41在食管癌细胞中的表达下调,EPB41过表达可通过抑制FAO途径来抑制食管癌细胞的增殖和迁移,这可能是通过调控Wnt/β-catenin信号通路转导来实现。Objective To investigate the effect of erythrocyte membrane protein band 4.1(EPB41)on the malignant phenotype of esophageal cancer cells and explore the possible underlying mechanisms based on fatty acid oxidation(FAO)mediated by the Wnt/β-catenin signaling pathway.Methods KYSE30 cells and KYSE150 cells in logarithmic growth phase were divided into 5 groups each.The mRNA and protein expression levels,cell proliferation and migration rate,ATP level,NADPH/NADP^(+)ratio,reactive oxygen species(ROS)and GSH levels were detected.Results In KYSE30 cells,the number of cell clones,cell migration rate,ATP level,NADPH/NADP^(+)ratio,ROS level,and protein expression levels of Wnt3a,β-catenin,and nuclearβ-catenin in cells were significantly increased in the EPB41 siRNA group(P<0.05),and fatty acid oxidation inhibitor and Wnt pathway inhibitor could reverse the effect of EPB41.In KYSE150 cells,the number of clones,cell migration rate,ATP level,NADPH/NADP^(+)ratio,ROS level,and the expression levels of Wnt3a,β-catenin,and nuclearβ-catenin proteins were significantly reduced in the cells in the EPB41 overexpression group(P<0.05),and the effect of EPB41 could be reversed by the fatty acid oxidation inducer and the Wnt pathway activator.Conclusion EPB41 expression is down-regulated in esophageal cancer cells.EPB41 overexpression can inhibit the proliferation and migration of esophageal cancer cells by suppressing the FAO pathway,which may be achieved by modulating the Wnt/β-catenin signaling transduction.

关 键 词：EPB41 食管癌 脂肪酸氧化 WNT/Β-CATENIN信号通路 

分 类 号：R392.11[医药卫生—免疫学]