miR-425-5p在子宫内膜癌中的诊断价值及其对子宫内膜癌细胞增殖、迁移和凋亡的影响  

Diagnostic Value of miR-425-5p in Endometrial Cancer and Effect of Inhibiting miR-425-5p on Proliferation,Migration and Apoptosis in Endometrial Cancer Cells

作　　者：白咪红 张洁[2] 寇莉[2] 王金波[1] BAI Mihong;ZHANG Jie;KOU Li;WANG Jinbo(Department of Laboratory,Baoji People’s Hospital,Baoji,Shaanxi,721000;Department of Obstetrics and Gynecology,Baoji People’s Hospital,Baoji,Shaanxi,721000)

机构地区：[1]宝鸡市人民医院检验科,陕西省宝鸡市721000 [2]宝鸡市人民医院妇科,陕西省宝鸡市721000

出　　处：《医学分子生物学杂志》2025年第2期173-179,共7页Journal of Medical Molecular Biology

基　　金：宝鸡市卫生健康科研项目(No.2023-096)。

摘　　要：目的分析miR-425-5p在子宫内膜癌(endometrial carcinoma,EC)中的诊断价值及其对EC细胞增殖、迁移和凋亡的影响。方法RT-qPCR检测EC、子宫良性病变患者血清和组织miR-425-5p表达水平,比较EC不同病理特征患者组织、血清miR-425-5p表达情况,分析组织、血清miR-425-5p与病理特征的关系及其对EC的诊断价值;RT-qPCR检测正常子宫内膜上皮hEEC细胞、子宫内膜癌(HEC-1A、Ishikawa、HEC-1B)细胞miR-425-5p表达水平。将Ishikawa细胞分为Con组(空白培养)、anti-miR-NC组(转染anti-miR-NC)、anti-miR-425-5p组(转染anti-miR-425-5p),RT-qPCR检测miR-425-5p表达,MTT和流式细胞术检测细胞增殖、周期和凋亡,Transwell检测细胞迁移,蛋白质印迹法检测CyclinD1、PCNA、Bax、Bcl-2、MMP9蛋白表达。结果EC组血清和组织中miR-425-5p表达水平高于良性病变组(P<0.05);不同淋巴结转移、FIGO分期、分化程度病理特征患者组织、血清miR-425-5p表达情况比较,差异有统计学意义(P<0.05);组织、血清miR-425-5p与FIGO分期、淋巴结转移、分化程度呈正相关(r=0.793、0.689、0.665、0.694、0.652、0.672,P<0.05),组织、血清miR-425-5p诊断EC的AUC分别为0.782、0.762,敏感度分别为74.36%、71.79%,特异度分别为71.79%、70.51%。与hEEC细胞相比,HEC-1A、Ishikawa、HEC-1B细胞中miR-425-5p表达水平升高(P<0.05);与anti-miR-NC组或Con组相比,anti-miR-425-5p组miR-425-5p表达水平、细胞活性、S期细胞比例、迁移细胞数、Bcl-2、MMP9、CyclinD1、PCNA蛋白表达明显降低,G_(0)/G_(1)细胞比例、凋亡率、Bax蛋白表达明显升高(P<0.05)。结论miR-425-5p在EC组织和血清中高表达,与FIGO分期、淋巴结转移、分化程度有关,对EC诊断具有一定价值,且抑制miR-425-5p能明显抑制EC细胞增殖和迁移,并诱导细胞凋亡。Objective To analyze the diagnostic value of miR-425-5p in endometrial cancer(EC)and the effect of inhibiting miR-425-5p on the proliferation,migration,and apoptosis of EC cells.Methods The expression level of miR-425-5p in serum and tissues of patients with EC and benign uterine lesions was detected by RT-qPCR,and was compared among patients with different pathological characteristics of EC.The relationship between miR-425-5p expression in the tissues and serum and the pathological characteristics was analyzed,as well as its diagnostic value for EC.The expression level of miR-425-5p in normal endometrial epithelial hEEC cells,endometrial carcinoma Ishikawa,HEC-1A,HEC-1B cells was detected by RT-qPCR.Ishikawa cells were divided into 3 groups:Con group(no treatment control),anti-miR-NC group(transfected with anti-miR-425-5p)and anti-miR-425-5p group(transfected with anti-miR-425-5p).RT-qPCR was used to detect the miR-425-5p expression level.MTT and flow cytometry were used to detect cell proliferation,cell cycle and apoptosis.cell migration was detected by Transwell.The expression levels of CyclinD1,PCNA,Bax,Bcl-2 and MMP9 were detected by Western blotting.Results The expression level of miR-425-5p in serum and tissues of the endometrial carcinoma group was higher than that of the benign lesion group(P<0.05).There were statistically significant differences when comparing the expression level of miR-425-5p in patients with different lymph node metastasis statuses,FIGO stages and degrees of differentiation(P<0.05).The expression of miR-425-5p in tissues and serum was positively correlated with FIGO stage,lymph node metastasis,and degree of differentiation(r=0.793,0.689,0.665,0.694,0.652,0.672,P<0.05).The AUC of miR-425-5p in tissues and serum for the diagnosis of EC were 0.782 and 0.762,respectively.The sensitivities were 74.36%,71.79%,specificity,and the specificities were 71.79%,70.51%,respectively.The expression level of miR-425-5p in endometrial carcinoma Ishikawa,HEC-1A,HEC-1B cells was increased when compared

关 键 词：miR-425-5p 子宫内膜癌 诊断 细胞增殖 细胞周期 细胞凋亡 

分 类 号：R446.6[医药卫生—诊断学]