七叶皂苷钠对高脂血症大鼠动脉粥样硬化和抗氧化活性的影响及作用机制  

Effect of Sodium Aescinate on Atherosclerosis and Antioxidant Activity in Hyperlipidemia Model Rats

作　　者：王学智[1] 郝亚逢[1] 原涛 许振坤[1] 高淑贤 WANG Xuezhi;HAO Yafeng;YUAN Tao;XU Zhenkun;GAO Shuxian(Department of Cardiology,the First Hospital of Handan,Handan,Hebei,056001,China)

机构地区：[1]邯郸市第一医院心内科,河北省邯郸市056001

出　　处：《医学分子生物学杂志》2025年第2期187-193,共7页Journal of Medical Molecular Biology

基　　金：河北省医学科学研究课题计划(No.20220426)。

摘　　要：目的探究七叶皂苷钠对高脂血症大鼠动脉粥样硬化和抗氧化活性的影响及其作用机制。方法将72只SD大鼠随机分为6组:对照组,模型组,七叶皂苷钠0.5、1、2 mg/kg组和阿托伐他汀钙10 mg/kg组。除对照组外,其余5组建立高脂血症大鼠模型。分组给予相应药物处理后,处死大鼠,制作心肌组织切片进行HE染色。检测血脂、全血黏度指标、氧化应激指标并计算动脉粥样硬化指数(AI)和抗动脉粥样硬化指数(HDL-C/TC)。蛋白质印迹检测各组大鼠Keap1-Nrf2/ARE信号通路相关蛋白表达。结果与模型组比较,七叶皂苷钠1、2 mg/kg组和阿托伐他汀钙10 mg/kg组大鼠心肌纤维排列较疏散,细胞核溶解及损伤程度明显改善,TC、TG、LDL-C水平降低,HDL-C水平升高,终末体重、AI降低,HDLC/TC水平升高,全血黏度高切、中切、低切及血浆黏度降低,ApoA-Ⅰ水平升高、ApoB100水平降低,SOD、GSH-Px升高,MDA降低,Keap1、NQO1、ARE和p-Nrf-2/Nrf-2蛋白表达水平显著升高(P<0.05)。结论七叶皂苷钠可改善高脂血症模型大鼠心肌组织病理损伤,调节血脂异常状态,抗动脉粥样硬化,抑制氧化应激,其作用机制可能与激活Keap1-Nrf2/ARE信号通路有关。Objective To investigate the effect of sodium aescinate on atherosclerosis and antioxidant activity in hyperlipidemia model rats and its mechanism.Methods Seventy-two SD rats were randomly divided into 6 groups:control group,model group,sodium aescinate 0.5 mg/kg group、1 mg/kg、2 mg/kg groups and atorvastatin calcium 10 mg/kg group.Except for the control group,hyperlipidemia rat model was established in the other five groups.After the corresponding drug treatments were administered to each group,the rats were sacrificed,and myocardial tissue sections were prepared for HE staining.The blood lipids,whole blood viscosity indicators,and oxidative stress indicators were detected by automatic biochemical analyzer,and the atherosclerosis index(AI)and index of HDL-C/TC were calculated.The expression of Keap1-Nrf2/ARE signaling pathway related proteins in rats were detected by using Western blotting.Results The myocardial fiber arrangement in the sodium aescinate 1 mg/kg and 2 mg/kg groups and the atorvastatin calcium 10 mg/kg group were more dispersed than that in the model group,the degree of nuclear lysis and damage were improved,the levels of TC,TG and LDL-C were decreased,the level of HDL-C was increased,the final body weight and AI were decreased,and the value of HDL-C/TC was increased when compared with those in the model group.The whole blood viscosity and the plasma viscosity were decreased,the level of ApoA-I and SOD and GSH-Px were increased,the level of ApoB100 and MDA was decreased,and the protein expression levels of Keap1,NQO1,ARE and p-Nrf-2/Nrf-2 were significantly increased(P<0.05).Conclusion Sodium aescinate can improve myocardial histopathological injury,regulate dyslipidemia,resist atherosclerosis and inhibit oxidative stress in hyperlipidemia model rats,and its mechanism may be related to the activation of Keap1-Nrf2/ARE signaling pathway.

关 键 词：高脂血症 心肌损伤 七叶皂苷钠 抗动脉粥样硬化 氧化应激 Keap1-Nrf2/ARE信号通路 

分 类 号：R285.5[医药卫生—中药学]