肺结核患者外周血miR-20a、NOD2 mRNA表达水平及其预后评估价值  

Expression levels of miR-20a and NOD2 mRNA in peripheral blood of patients with pulmonary tuberculosis and their prognostic value

作　　者：孙卓琳 李佐君[2] SUN Zhuolin;LI Zuojun(Department of Preventive Health Care,Beijing Tongren Hospital Affiliated to Capital Medical University,Beijing,China,100176;Department of Infection,Beijing Tongren Hospital Affiliated to Capital Medical University,Beijing,China,100176)

机构地区：[1]首都医科大学附属北京同仁医院预防保健科,北京100176 [2]首都医科大学附属北京同仁医院感染科,北京100176

出　　处：《分子诊断与治疗杂志》2025年第2期300-303,308,共5页Journal of Molecular Diagnostics and Therapy

基　　金：北京市自然科学基金(7232230)。

摘　　要：目的探究肺结核患者外周血微小RNA(miR)-20a、核苷酸结合寡聚化结构域蛋白2(NOD2)mRNA表达水平及其预后评估价值。方法选择2022年1月至2023年12月在北京同仁医院收治的178例肺结核患者为研究对象,患者经6个月标准化抗结核治疗后进行预后评估,根据预后情况分为预后良好组(n=136)及预后不良组(n=42)。比较两组患者血清miR-20a、NOD2 mRNA表达水平。采用Logistic回归分析影响肺结核患者预后的危险因素。绘制受试者工作特征(ROC)曲线评估血清miR-20a、NOD2 mRNA表达水平对肺结核患者预后不良的预测价值。结果两组治疗后的血清miR-20a mRNA表达水平均降低,而NOD2 mRNA表达水平则均升高,且预后良好组改善更优,差异有统计学意义(P<0.05)。与预后良好组相比,预后不良组血清miR-20a mRNA表达水平升高,差异有统计学意义(P<0.05);NOD2 mRNA表达水平降低,差异有统计学意义(P<0.05)。Logistic回归分析显示:年龄、肺部空洞、血清miR-20a、NOD2 mRNA表达水平是肺结核患者预后不良的独立危险因素(P<0.05)。ROC曲线分析显示,血清miR-20a、NOD2 mRNA单独及联合预测的曲线下面积分别为0.827,0.769,0.895,联合预测价值高于单独预测(P<0.05)。结论肺结核患者血清miR-20a mRNA表达水平升高,NOD2 mRNA表达水平降低,两者与预后结局密切相关,联合预测价值更高。Objective To investigate the mRNA expression levels of micrornas(Mir-20a)and nucleotide-binding oligomerization domain protein 2(NOD2)in peripheral blood of patients with pulmonary tuberculosis and their prognostic value.Methods A total of 178 patients with pulmonary tuberculosis treated at Beijing Tongren Hospital from January 2022 to December 2023 were selected for this study.After six months of standardized anti-tuberculosis treatment,the prognosis was assessed,and the patients were divided into two groups based on their prognosis:a good prognosis group(n=136)and a poor prognosis group(n=42).Two groups of patients were compared based on serum miR-20a and NOD2 mRNA expression levels.Logistic regres-sion was used to analyze the risk factors affecting the prognosis of patients with pulmonary tuberculosis.A receiver operating characteristic(ROC)curve was drawn to assess the predictive value of serum miR-20a and NOD2 mRNA expression in determining poor prognosis among patients with pulmonary tuberculosis.Results After treatment,the serum miR-20a mRNA expression level decreased in both groups,while the NOD2 mRNA expression level increased in both groups.The improvement was more significant in the group with a good prognosis,with statistical significance(P<0.05).In comparison to the good prognosis group,the serum miR-20a mRNA expression level in the poor prognosis group increased significantly(P<0.05),while NOD2 mRNA expression level decreased significantly(P<0.05).Logistic regression analysis showed that age,pulmonary cavity,serum miR-20a,and NOD2 mRNA expression levels were independent risk factors for poor prognosis in patients with pulmonary tuberculosis(P<0.05).ROC curve analysis indicated that the areas under the curve of serum miR-20a and NOD2 mRNA were 0.827,0.769 and 0.895 respectively.The combined predictive value was higher than that of single prediction(P<0.05).Conclusion The serum miR-20a mRNA expression level increased,while NOD2 mRNA expression level decreased in patients with pulmonary tuberculosis.Th

关 键 词：肺结核 微小RNA-20a 核苷酸结合寡聚化结构域蛋白2 

分 类 号：R73[医药卫生—肿瘤]