多浪羊与湖羊杂交产羔性状COIL及BMPR-IB基因遗传效应分析  

Analysis of genetic effects of COIL and BMPR-IB genes for lambing traits in Duolang sheep and Hu sheep crossbred sheep

作　　者：史香云 李焦智 刘玲玲[1] 刘武军[1] SHI Xiangyun;LI Jiaozhi;LIU Lingling;LIU Wujun(College of Animal Science,Xinjiang Agricultural University,Urumqi 830052,China;Wensu Chishichengyang Husbandry Co.,Ltd.,Wensu Xinjiang 843100,China)

机构地区：[1]新疆农业大学动物科学学院,乌鲁木齐830052 [2]温宿叱石成羊畜牧有限公司,新疆温宿843100

出　　处：《新疆农业科学》2025年第1期243-250,共8页Xinjiang Agricultural Sciences

基　　金：国家重点研发项目“南疆地方肉羊选育关键技术研究与示范”(2021YFD1600702-1)。

摘　　要：【目的】分析COIL及BMPR-IB基因与产羔数关联性,探究多浪羊与湖羊(简称多湖杂交)后代产羔性状COIL及BMPR-IB基因的遗传效应。【方法】研究以多浪羊与湖羊级进杂交F_(1)代、F_(2)代、F_(3)代为研究对象,随机选取多湖杂交F_(1)代(228只)、F_(2)代(254只)、F_(3)代(252只),进行全血DNA提取,COIL基因利用扩增阻滞突变系统PCR(ARMS-PCR)技术基因分型、BMPR-IB基因利用限制性内切酶片段长度多态性技术(PCR-RFLP)基因分型后,与产羔数关联分析。【结果】在多湖杂交F_(1)代中,突变纯合GG型产羔数极显著高于突变杂合CG、野生CC型产羔数(P<0.01),突变杂合CG型产羔数极显著高于野生CC型产羔数(P<0.01);在多湖杂交F_(2)代中突变纯合GG型与突变杂合CG、野生CC型产羔数间均差异极显著(P<0.01);在多湖杂交F_(3)代中,突变纯合GG、突变杂合CG型产羔数极显著高于CC型(P<0.01),GG型产羔数极显著高于CG型(P<0.05)。在多湖杂交F_(1)、F_(2)、F_(3)代中均为突变纯合BB型产羔数极显著高于突变杂合B+型、野生++型产羔数(P<0.01),突变杂合B+型产羔数极显著高于野生++型产羔数(P<0.01)。【结论】COIL基因与BMPR-IB基因均对多湖杂交后代产羔数影响显著,但BMPR-IB基因在多湖杂交后代突变率较低,可能不适合作为筛选多湖杂交双羔群体的关键基因,COIL基因可以作为筛选多湖杂交高繁殖率的新候选基因。【Objective】This study aims to investigate the genetic effects of lambing traits COIL and BMPR-IB genes in the offspring of Duolang sheep and Hu sheep crossbreeding(Duohu sheep crossbreeding)cascade crosses by analyzing the association between COIL and BMPR-IB genes and the number of lambs produced.【Methods】Randomly selected F_(1)(228),F_(2)(254),and F_(3)(252)generations of Duohu crossbred sheep were subjected to whole blood DNA extraction,and the COIL gene was genotyped using amplification refractory mutation system-PCR(ARMS-PCR)technique,and the BMPR-IB gene was analyzed in association with the number of lambs produced after genotyping using PCR-restriction fragment length polymorphism(PCR-RFLP)technique.【Results】In the Duohu F_(1)sheep,the lambing numbers of the mutant pure GG type were significantly higher than those of the mutant heterozygous CG and the wild CC type(P<0.01),and that of the mutant heterozygous CG type were significantly higher than that of the wild CC type(P<0.01);in the Duohu F_(2)sheep,the differences between the number of lambs produced by the mutant pure GG type and the mutant heterozygous CG and wild CC types were all highly significant(P<0.01);in the Duohu F_(3)sheep,the number of lambs produced by the mutant pure GG and the mutant heterozygous CG types was highly significantly higher than that of the CC type(P<0.01),and the number of lambs produced by the GG type was highly significantly higher than that of the CG type(P<0.05).In the Duohu F_(1),F_(2)and F_(3)sheep,the numbers of lambs produced by the mutant pure BB type were highly significantly higher than the number of lambs produced by the mutant heterozygous B+type and the wild++type(P<0.01),and the number of lambs produced by the mutant heterozygous B+type was highly significantly higher than that of the wild++type(P<0.01).【Conclusion】Both COIL gene and BMPR-IB gene have significant effect on lambing number of Duo and Hu crossbred sheep.However,the BMPR-IB gene has a low mutation rate in the progeny of Duohu sheep

关 键 词：多浪羊 湖羊 杂交 产羔性状 COIL基因 BMPR-IB基因 遗传效应 

分 类 号：S826.6[农业科学—畜牧学]