预接种根瘤菌提高野生大豆幼苗耐盐性与多胺代谢的关系  

Relationship between salt tolerance enhanced by pre-inoculation with rhizobium and polyamine metabolism in wild soybean seedlings

作　　者：黄巧玥 李陈亚 程聪 於丙军[1,2] HUANG Qiaoyue;LI Chenya;CHENG Cong;YU Bingjun(Laboratory of Plant Stress Biology,College of Life Sciences,Nanjing Agricultural University,Nanjing 210095,China;College of Life Sciences,Xinjiang Agricultural University,Urumqi 830052,China)

机构地区：[1]南京农业大学生命科学学院植物逆境生物学实验室,江苏南京210095 [2]新疆农业大学生命科学学院,新疆乌鲁木齐830052

出　　处：《南京农业大学学报》2025年第2期337-347,共11页Journal of Nanjing Agricultural University

基　　金：江苏省农业科技自主创新项目[CX(22)2003];新疆维吾尔自治区高校基本科研业务费项目(XJEDU2024Z001);新疆土壤与植物生态过程重点实验室开放课题(23XJTRZW16)。

摘　　要：[目的]本文旨在研究预接种根瘤菌提高野生大豆幼苗耐盐性过程中多胺代谢的变化,并探究其参与野生大豆幼苗盐胁迫适应的生理机制。[方法]以耐盐性不同的野生大豆BB52(耐盐)和N23227(盐敏感)材料为研究对象,采用预接种根瘤菌的方法,分析和比较盐胁迫下幼苗生长表型、光合参数和离子水平等形态和生理指标的差异。采用超高效液相色谱(UPLC)和实时荧光定量PCR(RT-qPCR)技术,探讨了预接种根瘤菌对盐胁迫下N23227幼苗根和叶游离态、结合态和束缚态多胺含量、多胺合成酶和氧化酶活性及合成酶编码基因表达水平的影响。[结果]预接种根瘤菌后的大豆幼苗盐害明显减轻,株高、干重和总氮含量,叶T_(r)、G_(s)和P_(n)及根、茎、叶K^(+)和NO_(3)^(-)含量明显恢复甚至超过对照水平,Na^(+)和Cl^(-)含量显著下降,其中N23227材料表现更明显,根、茎、叶Na^(+)/K^(+)和Cl^(-)/NO_(3)^(-)值比盐处理分别降低了43.86%、59.10%、38.42%和60.31%、76.72%、78.39%。盐胁迫下预接种根瘤菌的N23227幼苗根和叶多胺合成酶ADC活性较盐处理分别增加了59.29%和44.27%,根多胺分解酶二胺氧化酶(DAO)活性增加了20.00%,叶多胺氧化酶(PAO)活性增加了28.57%,Spd和Spm合成相关酶的编码基因GsSPMS、GsSPDS1和GsSAMDC4表达上调,促进了根和叶结合态、束缚态Spd和Spm的积累,(Spd+Spm)/Put值上升。[结论]预接种根瘤菌可增强野生大豆幼苗的耐盐性,可能通过调节根和叶多胺代谢酶活性及Spd和Spm合成相关酶编码基因的表达水平,促进内源结合态、束缚态Spd和Spm的积累,以维持较高的(Spd+Spm)/Put值,从而改善其在盐胁迫下的离子稳态、光合作用和生长状况。[Objectives]This study aimed to analyze the changes in polyamine metabolism during the enhancement of salt tolerance in wild soybean seedlings by pre-inoculation with Bradyrhizobium japonicum,in order to explore the physiological mechanisms of rhizobium inoculation participating in salt stress adaptation in wild soybean seedlings.[Methods]Using the wild soybean BB52(salt tolerant)and N23227(salt sensitive)as the experimental materials with pre-inoculation of rhizobia,the differences in morphological and physiological indexes such as seedlings growth phenotype,photosynthetic parameters,and ion contents under salt stress were analyzed and compared.On this basis,ultra performance liquid chromatography(UPLC)and quantitative real-time PCR(RT-qPCR)technology were used to explore the effects of pre-inoculation of rhizobia on the free,conjugated and bound polyamine contents,polyamine synthase and oxidase activities,and the expression levels of synthase-coding genes in the roots and leaves of N23227 seedling under salt stress.[Results]The salt injury of both wild soybean seedlings pre-inoculated with rhizobia was obviously mitigated,and the plant height,dry weight,total nitrogen content,and the values of leaf T_(r),G_(s) and P_(n),as well as the K^(+)and NO_(3)^(-) contents in roots,stems and leaves were apparently restored or even exceeded the control level.The accumulation of Na^(+)and Cl^(-)in roots,stems and leaves were significantly reduced,and among them,more prominent was showed in N23227,when compared with those of the NaCl treatment.The ratios of Na^(+)/K^(+)and Cl^(-)/NO_(3)^(-) in roots,stems and leaves decreased by 43.86%,59.10%,38.42%and 60.31%,76.72%and 78.39%,respectively.Furthermore,for the pre-inoculated N23227 seedlings under salt stress,the activities of polyamine synthetase arginine decarboxylase(ADC)in roots and leaves increased by 59.29%and 44.27%,the activities of polyamine catabolic enzymes diamine oxidase(DAO)in roots and polyamine oxidase(PAO)in leaves increased by 20.00%and 28.57%,respectively;a

关 键 词：野生大豆 盐胁迫 预接种根瘤菌 多胺代谢 (Spd+Spm)/Put值 离子稳态 

分 类 号：Q945.78[生物学—植物学]