接种具有N_(2)O减排效应的PGPR对大豆幼苗生长及土壤N_(2)O排放的影响  

Effects of inoculation with N_(2)O-mitigating PGPR strains on soybean seedling growth and N_(2)O emission from soil

作　　者：王洲章 朱津宏 高南 吴永红[3] 张振超[4] 吴国平[4] 申卫收 WANG Zhouzhang;ZHU Jinhong;GAO Nan;WU Yonghong;ZHANG Zhenchao;WU Guoping;SHEN Weishou(School of Environmental Science and Engineering/Jiangsu Provincial Key Laboratory of Atmospheric Environment Monitoring and Pollution Control/Jiangsu Provincial Collaborative Innovation Center of Atmospheric Environment and Equipment Technology,Nanjing University of Information Science and Technology,Nanjing 210044,China;College of Biological and Pharmaceutical Engineering/National Engineering Research Center for Biotechnology,Nanjing University of Technology,Nanjing 211816,China;State Key Laboratory of Soil and Sustainable Agriculture,Institute of Soil Science,Chinese Academy of Sciences,Nanjing 210008,China;Zhenjiang Institute of Agricultural Sciences,Hilly Region of Jiangsu,Zhenjiang 212400,China)

机构地区：[1]南京信息工程大学环境科学与工程学院/江苏省大气环境监测与污染控制高技术研究重点实验室/江苏省大气环境与装备技术协同创新中心,江苏南京210044 [2]南京工业大学生物与制药工程学院/国家生化工程技术研究中心,江苏南京211816 [3]中国科学院南京土壤研究所土壤与农业可持续发展国家重点实验室,江苏南京210008 [4]江苏丘陵地区镇江农业科学研究所,江苏镇江212400

出　　处：《南京农业大学学报》2025年第2期369-379,共11页Journal of Nanjing Agricultural University

基　　金：国家自然科学基金项目(32172673,42377311);江苏省重点研发计划(社会发展)项目(BE2020731)。

摘　　要：[目的]大豆是重要的粮油兼用作物,可在盐碱地、复垦土地等边际土地种植。本研究旨在明确具有N_(2)O减排效应的菌株对大豆生长和土壤N_(2)O排放的影响,进而研发具有促生减排效应的新型生物肥料。[方法]选取江苏省句容市的复垦土壤,通过室内盆栽试验,研究接种具有N_(2)O减排效应的菌株Azospirillum sp. TSA2S、Pseudomonas stutzeri NRCB010、Achromobacter denitrificans YSQ030和Bacillus subtilis NRCB002对大豆幼苗生长和土壤N_(2)O排放的影响。[结果]接种4株细菌显著提高大豆幼苗的株高、叶长、叶宽、地上部鲜重和地下部鲜重。接种菌株YSQ030、TSA2S、NRCB010和NRCB002后大豆幼苗地上部鲜重增加46%、45%、44%和35%(P<0.05),接种YSQ030、TSA2S和NRCB010大豆幼苗地下部鲜重增加65%、63%和26%(P<0.05)。接种4株植物根际促生菌(PGPR)显著减少大豆施用有机无机复混肥后土壤的N_(2)O排放,接种菌株NRCB002、NRCB010、TSA2S和YSQ030分别减少81.0%、79.0%、78.2%和78.0%(P<0.05)。接种PGPR菌株在盆栽试验处理2和14 d时显著降低土壤中nirK基因丰度。接种PGPR菌株显著增加土壤中nosZⅠ的基因丰度;接种菌株TSA2S、NRCB010和YSQ030显著增加nosZⅡ的基因丰度;接种菌株NRCB002在盆栽试验处理14 d时显著增加nosZⅡ的基因丰度。土壤N_(2)O排放量与土壤pH、nirS基因丰度呈显著正相关,但与nosZⅠ和nosZⅡ两个编码N_(2)O还原酶的基因呈极显著负相关。[结论]接种具有N_(2)O减排效应的菌株不仅可以促进大豆幼苗生长,还可以减少大豆施用有机无机复混肥后土壤的N_(2)O排放。[Objectives]Soybean is an important grain and oil crop and can be grown on marginal lands such as saline-alkali and reclaimed soils.The purpose of this study was to clarify the impact of bacterial strains with N_(2)O-mitigating effects on soybean growth and N_(2)O emissions from soil,and to develop a novel biofertilizer that promotes plant growth and reduced greenhouse gas emissions.[Methods]A reclaimed soil from Jurong City,Jiangsu Province,was selected,and the effects of inoculating four bacterial strains(Azospirillum sp.TSA2S,Pseudomonas stutzeri NRCB010,Achromobacter denitrificans YSQ030,and Bacillus subtilis NRCB002)with N_(2)O-mitigating effects on soybean seedling growth and N_(2)O emissions from soil were investigated through indoor pot experiments.[Results]The inoculation with the four bacterial strains significantly increased the plant height,leaf length,leaf width,shoot fresh weight,and root fresh weight of the soybean seedlings.The shoot fresh weight of the soybean seedlings increased by 46%,45%,44%and 35%(P<0.05)after inoculation with NRCB002,NRCB010,TSA2S and YSQ030,respectively.The root fresh weight of the soybean seedlings increased by 65%,63%and 26%(P<0.05)after inoculation with YSQ030,TSA2S and NRCB010.The inoculation with the four plant growth-promoting rhizobacteria(PGPR)significantly decreased N_(2)O emissions from the soil after the application of organic-inorganic compound fertilizer to soybeans.The N_(2)O emission were decreased by 81.0%,79.0%,78.2%and 78.0%after inoculation with YSQ030,TSA2S,NRCB010 and NRCB002(P<0.05).The inoculation with these four PGPR strains significantly decreased the abundance of the nirK gene in the soil after 2 and 14 days of treatment.The inoculation of PGPR strains significantly increased the abundance of the nosZⅠgene in the soil.Additionally,the inoculation of TSA2S,NRCB010 and YSQ030 significantly increased the abundance of the nosZⅡgene.So did the inoculation with NRCB002 after 14 days of treatment.N_(2)O emissions from soil were significantly positivel

关 键 词：生物肥料 温室气体减排 氮循环 植物根际促生菌(PGPR) N_(2)O还原细菌 

分 类 号：S144[农业科学—肥料学]