野生恒河猴肠道病毒病原谱分析  

Analysis of the Enterovirus Pathogen Spectrum in Wild Rhesus Macaques

作　　者：侯婧涵 徐鸿界 孙文亭 李明学 李艳艳[1,2] 靳玮华 杨凤梅 和占龙[1,2] HOU Jinghan;XU Hongjie;SUN Wenting;LI Mingxue;LI Yanyan;JIN Weihua;YANG Fengmei;HE Zhanlong(Institute of Medical Biology,Chinese Academy of Medical Sciences and Peking Union Medical College,Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Diseases,Kunming 650118,China;State Key Laboratory of Respiratory Health and Multimorbidity,Beijing 100005,China)

机构地区：[1]中国医学科学院/北京协和医学院医学生物学研究所,云南省重大传染病疫苗研发重点实验室,昆明650118 [2]呼吸与共病全国重点实验室,北京100005

出　　处：《实验动物科学》2025年第1期12-17,共6页Laboratory Animal Science

基　　金：国家重点研发计划(2021YFF0702804);中国医学科学院医学与健康科技创新工程(2021-I2M-1-024);中国医学科学院医学生物学研究所所级重点科研专项(2022IMBCAMS002);云南省重大科技专项计划(202002AA100009);北京协和医学院中央高校基本科研业务费(3332023079);云南省基础研究专项(202401CF070048)。

摘　　要：目的本研究旨在调查野生恒河猴的肠道病毒病原谱状况,为野生猴群疾病预防、引入及饲养管理人员职业健康防护提供科学依据。方法本研究收集云南省218份野生恒河猴新鲜粪便样本,提取病毒RNA,采用肠道病毒通用引物222/224、AN88/AN89进行巢式PCR检测,对阳性样本进行测序分析,以确认病毒种类。结果恒河猴肠道病毒携带率为11%(24/218),其中,婴猴组携带率为7.5%(4/53);青少年猴组携带率为3.5%(2/57);成年猴组携带率为16.7%(9/54);老年猴组携带率为16.7%(9/54)。结论经肠道病毒基因分型确定该肠道病毒病原谱主要为肠道病毒J组、肠道病毒sp.组和肠道病毒A组;经系统发育分析发现218只野生恒河猴肠道病毒病原谱与EVJ、EVA92、EVA119、SV19和SV46等亲缘关系较近。Objective This study aims to investigate the spectrum of enteric viruses in wild rhesus macaques,providing a scientific basis for the management and introduction of wild monkey populations,disease prevention,and occupational health protection for caregivers.Method This study collected 218 fresh fecal samples of wild rhesus macaques in Yunnan Province,fecal samples from rhesus macaques were collected,viral RNA was extracted,and RT-snPCR detection was performed using enterovirus universal primers 222/224 and AN88/AN89,and positive samples were sequenced and analyzed to confirm the virus type.Result This rhesus monkey group had an enterovirus carrier rate of 11%(24/218),as follows:baby monkeys had a carrier rate of 7.5%(4/53);young monkeys had a carrier rate of 3.5%(2/57);adult monkeys had a carrier rate of 16.7%(9/54);and the carrier rate of the old monkey group was 16.7%(9/54).Conclusion Genotyping of enteric viruses indicated that the virus spectrum primarily consisted of Enterovirus J,Enterovirus sp.and Enterovirus A groups.Phylogenetic analysis suggested that the virus spectrum is closely related to EVJ,EVA92,EVA119,SV19,and SV46 enteroviruses.

关 键 词：肠道病毒 野生恒河猴 年龄 性别 系统发育 

分 类 号：Q95-3[生物学—动物学]