采样方式对小鼠外周血样品质量的影响  

Influence of Sampling Methods on the Quality of Mouse Peripheral Blood Samples

作　　者：路婉婷 李夏 肖好好 佟巍[1] 郭智 张旭 董伟[1] 张奇 向志光[1] LU Wanting;LI Xia;XIAO Haohao;TONG Wei;GUO Zhi;ZHANG Xu;DONG Wei;ZHANG Qi;XIANG Zhiguang(Institute of Laboratory Animals Science,Chinese Academy of Medicalciences,Comparative Medicine Center,Peking Union Medical College,Beijing 100021,China;Beijing City University,Beijing 100094,China)

机构地区：[1]中国医学科学院医学实验动物研究所,北京协和医学院比较医学中心,北京100021 [2]北京城市学院,北京100094

出　　处：《实验动物科学》2025年第1期18-22,共5页Laboratory Animal Science

基　　金：十四五国家重点研发计划(2023YFF0724900)。

摘　　要：目的评价小鼠不同采血方式对血液样品质量的影响。方法对C57BL/6J和ICR两个品系的小鼠在眼眶静脉丛、下颌静脉和尾尖3个采血部位,以麻醉状态和未麻醉状态进行血液样本采集,分离血清和血浆样品;测定血液样本415、541、576 nm血红蛋白特征光吸收值评价溶血情况;以ELISA方法测定样品中皮质酮、促肾上腺皮质激素评估动物应激水平。结果通过尾尖采血方式获得的血液样本在415、541、576 nm的吸光度变异度大,部分样品在血红蛋白特征波长光吸收高,与其他两种方法存在统计学差异;不同采血方式获得的血清及血浆样品中促肾上腺皮质激素水平差异不显著;未麻醉动物眼眶静脉丛采集方法获取的血浆皮质酮含量高于下颌取血方法(P<0.05)。结论尾尖采血方式受操作稳定性影响,血液样品溶血风险高;下颌采血方式采集量较多,溶血风险小,较之眼眶采血方式不易引起动物应激。Objective To evaluate the influence of different blood collection method on the quality of blood samples in mice.Method For C57BL/6J and ICR mice,three blood collection sites were used:orbital venous plexus,mandibular vein and caudal tip,and blood samples were collected at anesthetic or not,and serum and plasma were separated,respectively.The light absorption of samples at 415,541 and 576 nm was measured to evaluate the hemolytic quality.ELISA was used to measure corticosterone and adrenocorticotropic hormone to evaluate the stress states of animals.Result The absorbance variability of the blood samples obtained by the tail tip blood collection method at 415 nm,541 nm and 576 nm was large,and the heme light absorption of some samples was high,which was statistically different from the other two method.There was no significant difference in the level of adrenocorticotropic hormone in serum and plasma samples obtained by different blood collection method,and the plasma corticosterone content obtained by the orbital venous plexus collection method of non-anesthetized animals was higher than that obtained by the mandibular blood collection method(P<0.05).Conclusion The blood samples from the tail tip are affected by the stability of operation,and there is a risk of hemolysis in blood samples.Mandibular blood collection is easy to operate,the collection volume is large,the blood collection time is short,the hemolysis risk is low and the level of irritation to animals is low than method by orbital venous plexus.

关 键 词：眼眶静脉丛采血 下颌静脉采血 尾尖采血 血红蛋白 皮质酮 促肾上腺皮质激素 

分 类 号：Q95-3[生物学—动物学]