单次大剂量照射SD大鼠肝后对血常规和肝功能的影响  

Effect of Blood Routine and Liver Function in SD Rats After Single High Dose Irradiation on Liver

作　　者：李文哲 黄裔玮 徐丽 黄裔闻 刘陈晨 李文军 张萌 袁芳 LI Wenzhe;HUANG Yiwei;XU Li;HUANG Yiwen;LIU Chenchen;LI Wenjun;ZHANG Meng;YUAN Fang(Department of Radiotherapy,General Hospital of Xinjiang Military Command,Wulumuqi 830000,China;Department of Clinical Laboratory,General Hospital of Xinjiang Military Command,Wulumuqi 830000,China)

机构地区：[1]中国人民解放军新疆军区总医院放疗科,乌鲁木齐830000 [2]中国人民解放军新疆军区总医院检验科,乌鲁木齐830000

出　　处：《实验动物科学》2025年第1期45-50,共6页Laboratory Animal Science

基　　金：新疆维吾尔自治区自然科学基金(2021D01C471)。

摘　　要：目的本研究探索射线单次大剂量照射后对SD大鼠腹腔静脉血血常规和肝功能指标的影响。方法48只SD大鼠随机分为6组:正常对照组(C组)、照射前组(R0组)、照射3 d组(R1组)、照射7 d组(R2组)、照射14 d组(R3组)、照射21 d组(R4组)和照射28 d组(R5组)。观察记录各组大鼠生活情况和体质量变化。利用直线加速器6 MV 20 Gy大剂量单次照射大鼠部分肝,照射后取腹腔静脉血检测血常规和肝功能指标。结果照射组大鼠体质量先降低后升高,各组体质量均小于同时间C组大鼠体质量,且差异有统计学意义(P<0.05)。血常规检测中白细胞(WBC)、血小板(PLT)和PEO(嗜酸性粒细胞百分数)检测结果与C组差异有统计学意义(P<0.05),肝功能检测中ALT、AST在照射后14 d,即R3组检测结果与C组对应指标差异有统计学意义(P<0.05)。照射前后复合组检测结果中,仅有HGB和BA差异无统计学意义。光镜下7 d后可观察到肝细胞水肿,随着时间增加,损伤程度加重。结论SD大鼠部分肝接受大剂量X线照射后,血常规和肝功能指标出现明显改变。光镜下组织病理学改变可能要早于血常规和肝功能检测指标的变化。Objective To explore effects of abdominal venous blood routine and liver function indicators in SD rats after a single high-dose radiation exposure.Method 48 SD rats were randomly divided into 6 groups:before irradiation(group R0),3 days after irradiation(group R1),7 days after irradiation(group R2),14 days after irradiation(group R3),21 days after irradiation(group R4),28 days after irradiation(group R5).The normal control groups(group C)correspond to the time of each R group.The partial liver of rats in the R group was given a single dose of 20 Gy 6 MV X-ray irradiation with a linear accelerator.After irradiation,weight changes of each group of rats were recorded and the blood routine and liver function indicators were test using abdominal venous blood.Result After a short decrease,the body weight of the irradiation group rats increased,the body weight of all groups were significantly smaller than that of group C at the same period(P<0.05).The result of white blood cells(WBC),platelets(PLT),and percentage of eosinophils(PEO)in routine blood tests showed significant differences compared to group C(P<0.05).14 days after irradiation,ALT and AST showed significant differences between group R3 and group C.in the corresponding indicators in liver function tests(P<0.05).The composite group test result showed that only HGB and PBA data have no significant difference before and after irradiation.At 7 days after irradiation,early pathological changes in the liver cells can be observed by light microscope.Moreover,with prolongation of the time,the injury was aggravated.Conclusion After high-dose X-ray irradiation of a portion of liver,blood routine and liver function data showed significant changes.However,pathological changes observed by light microscope could be prior to result of the blood routine and liver function test.

关 键 词：大剂量照射 SD大鼠 血常规 肝功能 肝 

分 类 号：Q95-3[生物学—动物学]