屏障环境中IVC系统内使用脏垫料哨兵小鼠监测螨虫和鞭毛虫感染  

Surveillance of Mite and Flagellate in Fection in Sentinel Mice Using Soiled Bedding in an IVC System in a Barrier Environment

作　　者：王美芳 于小亚 李志满 孙广杰 孙占微 杨永广[1,3] 孙丽光 WANG Meifang;YU Xiaoya;LI Zhiman;SUN Guangjie;SUN Zhanwei;YANG Yongguang;SUN Liguang(Laboratory Animal Center of the First Hospital,Jilin University,Changchun 130021,China;Jilin Science and Technology Innovation Platform Management Center,Changchun 130021,China;National-Local Joint Engineering Laboratory of Animal Models for Human Diseases,Changchun 130021,China)

机构地区：[1]吉林大学第一医院实验动物中心,长春130021 [2]吉林省科技创新平台管理中心,长春130021 [3]人类疾病动物模型国家地方联合工程实验室,长春130021

出　　处：《实验动物科学》2025年第1期69-73,共5页Laboratory Animal Science

摘　　要：目的验证屏障环境中的IVC系统内不同类型(玉米芯和刨花)脏垫料条件下的哨兵小鼠监测螨虫和鞭毛虫感染的有效时间和敏感度,以探讨其实际监测能力。方法将42只哨兵小鼠(4周龄雌性ICR小鼠)随机分为6组,间接添加螨虫和鞭毛虫双阳性小鼠的100%饲养脏垫料(玉米芯或刨花)给哨兵小鼠使用,或直接放置哨兵小鼠到螨虫和鞭毛虫双重感染的小鼠饲养笼内,暴露不同时间后检测哨兵小鼠的感染情况。结果6周后,添加玉米芯和刨花脏垫料的哨兵小鼠螨虫感染率分别为为20%和60%,但两组哨兵鼠鞭毛虫检测均为阴性。8周后,添加玉米芯和刨花脏垫料的哨兵小鼠螨虫感染率分别为33.33%和83.33%,但只有玉米芯饲养组鞭毛虫呈阳性(感染率为16.67%)。与螨虫和鞭毛虫双阳性小鼠共饲养在同一个笼内的哨兵小鼠在接触25 d时100%感染了螨虫和鞭毛虫。结论玉米芯脏垫料暴露在监控螨虫感染时不及刨花脏垫料敏感,提示刨花脏垫料暴露法对于种群不容易扩大的鼠群有借鉴意义。Objective To investigate the effective time and sensitivity of sentinel mice in monitoring mite and flagellate infection in IVC System in barrier environment under different types of soiled bedding(corncob and shavings),and to explore the actual monitoring ability of sentinel mice.Method 42 sentinel mice(4-week-old female ICR)were randomly divided into 6 groups.The sentinel mice were raised with 100%of the feeding soiled bedding(corncob or shavings)from mite and flagellate double positive mice,or directly placed in the cage of the mice infected with both mites and flagellates.Then the sentinel mice were detected at different times after exposure.Result After 6 weeks,the mite infection rates of the sentinel mice added soiled corncob and shavings bedding were 20%and 60%,respectively,but the detection of flagellate in both groups was negative.After 8 weeks,the mite infection rate of sentinel mice with 100%soiled corncob bedding was 33.33%,and the flagellate infection rate was 16.67%;the mite infection rate of sentinel mice with 100%soiled shaving bedding was 83.33%and no flagellate was detected.The sentinel mice,which were directly raised with mite and flagellate double infected mice in the same cage,were 100%infected with mites and flagellates on day 25.Conclusion The corncob soiled bedding is less sensitive than shavings soiled bedding in monitoring mite infection,suggesting that shavings soiled bedding exposure method has reference significance for mice whose population is not easily expanded.

关 键 词：哨兵小鼠 屏障环境 IVC系统 寄生虫感染 

分 类 号：Q95-3[生物学—动物学]