厦门近海不同年际间黄鳍棘鲷遗传多样性研究  

Genetic characteristics of Acanthopagrus latus among generationsin the coastal waters of Xiamen,Fujian Province

作　　者：方妍 陆晨阳 王艺红[3] 刘贤德[1] 胡战彪 蔡紫紫 李渊 张静[1,4] FANG Yan;LU Chenyang;WANG Yihong;LIU Xiande;HU Zhanbiao;CAI Zizi;LI Yuan;ZHANG Jing(Fisheries College,Jimei University,Xiamen 361021,China;Third Institute of Oceanography,Ministry of Natural Resources,Xiamen 361005,China;Zhangzhou Fisheries Technical Extension Station,Zhangzhou 363000,China;Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-environment,Xiamen 361021,China)

机构地区：[1]集美大学水产学院,福建厦门361021 [2]自然资源部第三海洋研究所,福建厦门361005 [3]漳州市水产技术推广站,福建漳州363000 [4]福建省海洋渔业资源与生态环境重点实验室,福建厦门361021

出　　处：《渔业研究》2025年第2期223-229,共7页Journal of Fisheries Research

基　　金：福建省科技厅引导性项目(2023N0011);福建省海洋渔业资源与生态环境重点实验室开放基金项目(Z822279);自然资源部第三海洋研究所基本科研业务费专项(海三科2024016)。

摘　　要：【背景】在全球渔业资源管理中,增殖放流作为一种重要的资源恢复措施被广泛应用,然而其对目标物种遗传特征的长期影响仍需深入研究。黄鳍棘鲷(Acanthopagrus latus)作为厦门近海重要的商业鱼类,其遗传特征的稳定性对于维护其种群健康至关重要。【目的】本研究旨在评估近年来增殖放流对厦门近海黄鳍棘鲷遗传特征的影响,了解其是否对种质资源产生显著影响。【方法】本研究采用线粒体DNA控制区序列对不同时间点采集的厦门近海黄鳍棘鲷样本进行测序,并利用遗传多样性分析、系统发育树构建及分子方差分析(AMOVA)等方法评估群体的遗传变异情况。【结果】分析显示,3个黄鳍棘鲷群体共获得66条线粒体DNA控制区序列,存在82个变异位点,共定义了58个单倍型,其中特有单倍型54个,黄鳍棘鲷群体的整体遗传多样性水平较高;基于58个黄鳍棘鲷线粒体控制区单倍型构建的NJ系统发育树显示,所有的黄鳍棘鲷聚类为一支;AMOVA分析显示,不同年份黄鳍棘鲷的遗传变异主要来自群体内,群体间遗传变异仅占1.23%。【结论】近年来厦门近海黄鳍棘鲷的增殖放流活动未对遗传多样性造成显著影响,群体遗传结构保持稳定。这一发现为未来增殖放流策略的优化和黄鳍棘鲷资源的可持续管理提供了支持。[Background]Stock enhancement has been widely used as an important resource restoration measurein global fisheries management.However,its long-term impact on the genetic characteristics of target speciesstill requires further investigation.Acanthopagrus latus,an important commercial fish species in the coastal wa-ters of Xiamen,Fujian Province,has genetic stability that is crucial for maintaining the health of its population.[Objective]This study aims to evaluate the impact of recent stock enhancement on the genetic characteristicsof A.latus in the coastal waters of Xiamen and to determine whether it has caused any significant effects on thegermplasm resources of the population.[Methods]Mitochondrial DNA control region sequences were used tosequence samples of A.latus collected at different time points in the coastal waters of Xiamen.Genetic di-versity analysis,phylogenetic tree construction,and analysis of molecular variance(AMOVA)were employedto assess the genetic variation within the populations.[Results]The analysis revealed 66 mitochondrial DNAcontrol region sequences obtained from 3 A.latus populations,with 82 polymorphic sites,defining 58 haplo-types,54 of which were unique.The overall genetic diversity level of the populations was high.The NJ-Treebased on 58 mitochondrial control region haplotypes showed that all A.latus clustered into one group.AMOVAanalysis indicated that the majority of genetic variation in the populations came from within the groups,withonly 1.23%of the variation attributed to differences between groups.[Conclusion]The recent stock enhance-ment activities in the coastal waters of Xiamen have not significantly affected the genetic diversity of A.latus,and the population’s genetic structure remains stable.This finding supports the optimization of future stock en-hancement strategies and the sustainable management of A.latus resources.

关 键 词：厦门近海 黄鳍棘鲷 增殖放流 遗传多样性 遗传结构 种质资源 

分 类 号：S937.3[农业科学—渔业资源]