Endophytic bacteria in different tissue compartments of African wild rice(Oryza longistaminata)promote perennial rice growth  

作　　者：Rui Tang Qinglin Tian Shuang Liu Yurui Gong Qingmao Li Rui Chen Linglin Wang Fengyi Hu Liyu Huang Shiwen Qin 

机构地区：[1]Key Laboratory of Biology and Germplasm Innovation of Perennial Rice,Ministry of Agriculture and Rural Affairs/School of Agriculture,Yunnan University,Kunming 650091,China

出　　处：《Journal of Integrative Agriculture》2025年第3期1001-1016,共16页农业科学学报(英文版)

基　　金：supported by funding from the National Natural Science Foundation of China(32060593 and 32060474);the Yunnan Provincial Science and Technology Department;China(202101AT070021 and 202101AS070001);the Yunnan Provincial Department of Education Science Research Fund Project;China(2023J0006);the Graduate Innovation Project of Yunnan University;China(KC-22223012 and ZC-22222760)。

摘　　要：Oryza longistaminata is an African wild rice species with valuable agronomic traits and the donor parent of perennial rice.Endophytic bacteria play an important role in host health,adaptive evolution and stress tolerance.However,endophytic bacterial communities in O.longistaminata and their plant growth-promoting(PGP)effects on the perennial rice of O.longistaminata offspring are poorly understood.In this study,the endophytic bacterial diversity,composition and network structures in the root,stem,and leaf tissues of O.longistaminata were characterized using Illumina sequencing of the 16S rRNA gene.The results suggested that O.longistaminata contains a multitude of niches for different endophytic bacteria,among which the root endosphere is more complex and functionally diverse than the stem and leaf endospheres.Tissue-specific biomarkers were identified,including Paludibaculum,Pseudactinotalea and Roseimarinus and others,for roots,Blautia for stems and Lachnospiraceae NK4A136 for leaves.The endophytic bacterial network of O.longistaminata was reassembled for various functions,including degradation/utilization/assimilation,detoxification,generation of precursor metabolites and energy,glycan pathways,macromolecule modification and metabolism.A total of 163 endophytic bacterial strains with PGP traits of potassium release,phosphate solubilization,nitrogen fixation,siderophore activity,indole-3-acetic acid(IAA)production,and 1-aminocyclopropane-1-carboxylate(ACC)deaminase activity were isolated from O.longistaminata.Eleven strains identified as Enterobacter cloacae,Enterobacter ludwigii,Stenotrophomonas maltophilia,Serratia fonticola,and Bacillus velezensis showed stable colonization abilities and PGP effects on perennial rice seedlings.Inoculated plants generally exhibited an enhanced root system and greater photosynthesis,biomass accumulation and nutrient uptake.Interestingly,two strains of E.cloacae have host genotype-dependent effects on perennial rice growth.The results of this study provide insights into the end

关 键 词：Oryza longistaminata endophytic bacteria plant growth-promotion perennial rice BIOFERTILIZER 

分 类 号：S511[农业科学—作物学]