TaFLZ54D enhances salt stress tolerance in wheat by interacting with TaSGT1 and TaPP2C  

作　　者：Yuxiang Qin Bao Zhang Shoufu Cui Xiaochun Qin Genying Li 

机构地区：[1]School of Biological Science and Technology,University of Jinan,Jinan 250022,China [2]Crop Research Institute,Shandong Academy of Agricultural Sciences,Jinan 250100,China

出　　处：《Journal of Integrative Agriculture》2025年第3期1017-1029,共13页农业科学学报(英文版)

基　　金：supported by the National Natural Science Foundation of China(31871622);the Key R&D Program of Shandong Province,China(2022LZG001)。

摘　　要：FCS-like zinc finger(FLZ)gene family members are C2-C2 zinc finger proteins that take part in seed dormancy,resistance to Myzus persicae 1,sucrose signaling and abiotic stresse tolerance.However,their functions,especially the molecular mechanism through which FLZs function,are not well understood.In this study,we characterized 120FLZs in wheat and revealed the function and mechanism of TaFLZ54D increasing salt stress tolerance in transgenic wheat.Expression analysis demonstrated that TaFLZ54D can be induced by NaCl treatment and it had the highest expression level under NaCl treatment among the 120 FLZs.Over-expression of TaFLZ54D increased wheat salt stress tolerance and the transgenic plants had higher levels of superoxide dismutase(SOD)and peroxidase(POD)activities and soluble sugar content,but a lower Na^(+)/K^(+)ratio and malondialdehyde(MDA)content than the wild type(WT)plants.Potassium ion transmembrane transporters and serine/threonine kinase inhibitor proteins showed differential expression between Ta FLZ54D transgenic wheat and the WT.Yeast two hybrid and luciferase complementation assays revealed that TaSGT1 and TaPP2C are the proteins that interact directly with TaFLZ54D.In summary,TaFLZ54D enhances salt stress tolerance through interaction with TaSGT1 and TaPP2C to reduce Na^(+)absorption and mitigate oxidative stress.The interaction between TaFLZ54D and TaSGT1,as well as TaPP2C indicated a link between salt stress tolerance of TaFLZ54D and the ubiquitin-mediated degradation of negative regulatory proteins.

关 键 词：WHEAT FLZ salt tolerance protein interaction Na^(+)exclusion 

分 类 号：S512.1[农业科学—作物学]