FS2 encodes an ARID-HMG transcription factor that regulates fruit spine density in cucumber  

作　　者：Hui Du Yue Chen Liangrong Xiong Juan Liu Keyan Zhang Ming Pan Haifan Wen Huanle He Run Cai Junsong Pan Gang Wang 

机构地区：[1]School of Agriculture and Biology,Shanghai Jiao Tong University,Shanghai 200240,China [2]Department of Plant Science and Technology,Shanghai Vocational College of Agriculture and Forestry,Shanghai 201699,China [3]Shanghai Agricultural Technology Extension and Service Center,Shanghai 201103,China [4]Shanghai Collaborative Innovation Center of Agri-Seeds,Shanghai Jiao Tong University,Shanghai 200240,China

出　　处：《Journal of Integrative Agriculture》2025年第3期1080-1091,共12页农业科学学报(英文版)

基　　金：supported by the National Natural Science Foundation of China(31972425);the Shanghai Agriculture Applied Technology Development Program,China(2020-02-08-00-08-F0148)。

摘　　要：Fruit spine density is an important commercial trait for cucumber(Cucumis sativus L.).Most North China-type cucumbers that are grown over large areas have a dense-spine phenotype,which directly affects the appearance quality,storage,and transportation of the fruits.Here,we isolated a novel few spines mutant(fs2)from the wild-type(WT)inbred line WD1,a North China-type cucumber with high density fruit spines,by an ethyl methanesulfonate(EMS)mutagenesis treatment.Genetic analysis revealed that the phenotype of fs2 is controlled by a single recessive nuclear gene.We fine-mapped the fs2 locus using F_(2) and BC_(1) populations(1,802 and 420 individuals,respectively),which showed that the candidate gene of FS2(Csa4G652850)encodes an ARID-HMG transcription factor containing an AT-rich interaction domain(ARID)and a high mobility group box domain(HMG).One SNP(C to T)and one InDel(a 40-bp deletion)in the coding region of FS2 result in amino acid variation and premature translation termination in the fs2 mutant,respectively.FS2 was found to be highly expressed in the apical buds and young ovaries.In addition,experiments suggest that FS2 participates in the regulation of fruit spine initiation by activating the expression of the Tril gene in cucumber.This work provides not only an important reference for understanding the molecular mechanisms of fruit spine development but also an important resource for fruit appearance quality breeding in cucumber.

关 键 词：CUCUMBER few spines FS2 TRICHOME ARID-HMG transcription factor 

分 类 号：S64[农业科学—蔬菜学]