Mitotic pollen abnormalities are linked to Ogura cytoplasmic male sterility in Chinese cabbage(Brassica rapa L.ssp.pekinensis)  

作　　者：Xiaochun Wei Yuanlin Zhang Yanyan Zhao Weiwei Chen Ujjal Kumar Nath Shuangjuan Yang Henan Su Zhiyong Wang Wenjing Zhang Baoming Tian Fang Wei Yuxiang Yuan Xiaowei Zhang 

机构地区：[1]Institute of Vegetables,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China [2]School of Agricultural Sciences,Zhengzhou University,Zhengzhou 450001,China [3]Department of Genetics and Plant Breeding,Bangladesh Agricultural University,Mymensingh 2202,Bangladesh

出　　处：《Journal of Integrative Agriculture》2025年第3期1092-1107,共16页农业科学学报(英文版)

基　　金：supported by the China Agricultural Research System(CARS-23-G15);the Innovation Team of Henan Academy of Agricultural Sciences,China(2024TD06);the Autonomous Innovation Project of Henan Academy of Agricultural Sciences,China(2024ZC034);the Joint Research on Agricultural Variety Improvement of Henan Province,China(2022010504);the Key Research&Development Project of Henan Province,China(221111110100)。

摘　　要：Ogura cytoplasmic male sterility(Ogura CMS)was first identified in wild radish(Raphanus sativus)and resulted in complete pollen abortion.However,the molecular mechanism of Ogura CMS in Chinese cabbage remains unclear.A cytological analysis confirmed nuclear degradation during the late uninucleate stage of pollen development,which diminished by the tricellular stage.Concurrently,tapetal cells exhibited abnormal enlargement and vacuolation starting from the tetrad stage.Serious developmental defects were observed in the pollen wall.During early pollen development,genes associated with cytochrome c and programmed cell death(PCD)were upregulated in the Ogura CMS line,while genes involved in pollen wall mitosis were downregulated.Conversely,at the late stage of pollen development,peroxisome and autophagy-related genes in the Ogura CMS line were upregulated.The mitochondrial orf138 gene mutation triggered the PCD process in tapetal cells,leading to their abnormal enlargement and the degradation of their contents,eventually resulting in vacuolation at the tricellular stage.These tapetal defects hindered the provision of adequate sporopollenin and nutrients to the microspores,consequently leading to abnormal pollen wall development and abnormal mitosis in the microspores.Ultimately,nuclear dispersion commenced during the late uninucleate stage,and autophagy occurred in the late stage of pollen development.Consequently,the plant could not produce functional pollen,resulting in male sterility in Chinese cabbage.Studies of Ogura CMS can promote the production and application of male sterile materials and enrich male sterile resources,which is of great significance for hybrid breeding.

关 键 词：Ogura CMS Chinese cabbage CYTOLOGY transcriptome PCD pollen abortion 

分 类 号：S634.1[农业科学—蔬菜学]