黄芩素调节磷脂酰肌醇3激酶/蛋白激酶B信号通路对先兆流产模型大鼠妊娠结局的影响  

Effect of baicalein regulating phosphatidylinoinosiol 3 kinase/protein kinase B signaling pathway on pregnancy outcome in threatened abortion model rats

作　　者：聂露[1] 吴荣华[1] 卢晓芳 Nie Lu;Wu Ronghua;Lu Xiaofang(Department of Emergency,Jiangxi Maternal and Child Health Hospital,Nanchang 330006)

机构地区：[1]江西省妇幼保健院急诊科,南昌330006

出　　处：《现代妇产科进展》2025年第3期196-200,共5页Progress in Obstetrics and Gynecology

摘　　要：目的:探究黄芩素(Bai)对先兆流产模型大鼠妊娠结局及对磷脂酰肌醇3激酶/蛋白激酶B(PI3K/Akt)信号通路的影响。方法:将雌性孕鼠随机分为对照(Control)组、模型(Model)组、Bai低剂量(Bai-L)组、Bai高剂量(Bai-H)组、阳性对照黄体酮组(PROG组)和Bai高剂量+PI3K抑制剂LY294002(Bai-H+LY294002)组,除Control组外其余均构建先兆流产模型,计算各组大鼠流产率。采用HE染色检测各组大鼠子宫组织结构病理变化;酶联免疫吸附法(ELISA)法检测各组大鼠血清干扰素γ(IFN-γ)、肿瘤坏死因子α(TNF-α)、白细胞介素10(IL-10)以及β-人绒毛膜促性腺激素(β-HCG)、孕激素、雌二醇等激素表达水平。Western blot法检测子宫组织中PI3K/Akt信号通路相关蛋白表达水平。结果:相较于Control组,Model组大鼠子宫组织结构出现损伤,细胞排列疏松,间质致密,内膜伴有炎性浸润,并且流产率以及血清中IFN-γ、TNF-α及IL-10表达水平显著升高(P<0.05),而雌二醇、β-HCG、孕激素水平和子宫组织中p-PI3K/PI3K和p-Akt/Akt值显著降低(P<0.05)。相较于Model组,Bai-L组、Bai-H组大鼠子宫组织结构损伤程度及内膜炎性浸润程度减轻,细胞排列逐渐趋于有序,流产率以及血清中IFN-γ、TNF-α及IL-10表达水平逐渐降低(P<0.05),雌二醇、β-HCG、孕激素水平和子宫组织中p-PI3K/PI3K和p-Akt/Akt值逐渐升高(P<0.05),且以上指标值Bai-H组与PROG组无显著差异(P>0.05);而PI3K抑制剂LY294002逆转了Bai对以上指标的影响(P<0.05)。结论:Bai能改善先兆流产大鼠的妊娠结局,其作用机制可能与激活PI3K/Akt信号通路有关。Objective:To investigate the effect of baicalein(Bai)on pregnancy outcomes and phosphatidylinositol 3-kinase/protein kinase B(PI3K/Akt)signaling pathway in rats with threatened miscarriage.Methods:Female pregnant mice were randomly grouped into Control group,Model group,Bai low-dose(Bai-L)group,Bai high-dose(Bai-H)group,positive control progesterone group(PROG group),and Bai high-dose+PI3K inhibitor LY294002(Bai-H+LY294002)group.Except for the Control group,the threatened miscarriage model was constructed.The miscarriage rate of rats in each group was calculated.HE staining was used to detect the pathological changes of uterine tissue structure in each group.Enzyme-linked immunosorbent assay(ELISA)was used to detect the expression levels of serum interferon-γ(IFN-γ),tumor necrosis factor-α(TNF-α),interleukin-10(IL-10),β-human chorionic gonadotropin(β-HCG),progesterone,estradiol and other hormones in each group.Western blot was used to detect the expression level of PI3K/Akt signaling pathway-related proteins in uterine tissue.Results:Compared with the Control group,the uterine tissue structure of rats in the Model group was damaged,with loose cell arrangement,dense stroma,and inflammatory infiltration in the endometrium,the miscarriage rate and the expression levels of IFN-γ,TNF-αand IL-10 in serum were greatly increased(P<0.05),the levels of estradiol,β-HCG,progesterone,and the p-PI3K/PI3K and p-Akt/Akt in uterine tissue were greatly decreased(P<0.05).Compared with the Model group,the degree of uterine tissue structure damage and endometrial inflammatory infiltration in the Bai-L group and Bai-H group were reduced,and the cell arrangement gradually tended to be orderly,the miscarriage rate and the expression levels of IFN-γ,TNF-αand IL-10 in serum were gradually decreased(P<0.05),the levels of estradiol,β-HCG,progesterone,and the p-PI3K/PI3K and p-Akt/Akt in uterine tissue were gradually increased(P<0.05).There was no great difference between the Bai-H group and the PROG group(P>0.05).The addition of

关 键 词：先兆流产 黄芩素 磷脂酰肌醇3激酶/蛋白激酶B信号通路 妊娠结局 

分 类 号：R714.2[医药卫生—妇产科学]