微瓦量级极低强度297 nm窄带LED对幼兔角膜损伤初探  

Preliminary Study on The Damage Effect of 297 nm Narrow-band LED Irradiation at Extremely Low Light Intensity of Microwatt on The Cornea of Infancy Rabbits

作　　者：陈东亚 谭一舟 谯煜 张美慧 顾瑛 CHEN Dongya;TAN Yizhou;QIAO Yu;ZHANG Meihui;GU Ying(Medical School of Chinese PLA,Beijing 100853,China;Department of Laser Medicine,the First Medical Center of Chinese PLA General Hospital;Laser Medicine Center,Hainan Hospital,Chinese PLA General Hospital;Department of Pathology,Hainan Hospital,Chinese PLA General Hospital;Precision Laser Medical Diagnosis and Treatment Innovation Unit,Chinese Academy of Medical Sciences)

机构地区：[1]解放军医学院,北京市100853 [2]中国人民解放军总医院第一医学中心激光医学科 [3]中国人民解放军总医院海南医院激光医学中心 [4]中国人民解放军总医院海南医院病理科 [5]中国医学科学院精准激光医学诊疗创新单元

出　　处：《中国激光医学杂志》2024年第6期308-312,358,共6页Chinese Journal of Laser Medicine & Surgery

基　　金：国家重点研发计划项目(2017YFB0403801);中国医学科学院医学与健康科技创新工程项目资助(2019-I2M-5-061)。

摘　　要：目的探究微瓦量级极低强度波长297nm窄带发光二极管(Light-emitting diode,LED)单次照射幼兔角膜的损伤阈值及损伤病理特点。方法研究对象为中华黑兔7只14只眼,按照照射功率密度不同分为3个大组:5μW/cm^(2)组(A组)、10μW/cm^(2)组(B组)、15μW/cm^(2)组(C组),每个功率密度组再根据能量密度不同分为18 mJ/cm^(2)组(A1组、B1组、C1组)、24 mJ/cm^(2)组(A2组、B2组、C2组)、30 mJ/cm^(2)组(A3组、B3组、C3组)、36 mJ/cm^(2)组(A4组、B4组、C4组)共4个亚组,对照组2只眼,实验组每个光参量组1只眼,各组均采用波长(297±5)nm的窄带LED光源单次照射兔眼角膜。光照后每隔6 h使用裂隙灯观察角膜有无浑浊,24 h后行荧光素钠染色评估记录角膜损伤部位、面积,深度,作为判定损伤阈值依据。并行眼球活检取材,HE染色评估角膜组织病理学变化。结果波长297 nm窄带LED光源功率密度5μW/cm^(2)、10μW/cm^(2)、15μW/cm^(2)单次照射幼兔角膜,出现损伤阈值分别为30、24、18 mJ/cm^(2)。损伤阈值及以上剂量照射角膜后24 h,荧光素钠染色结果为角膜瞳孔区出现点状、片状染色;HE染色观察,角膜上皮层细胞分布稀疏,排列紊乱,基底层细胞肿胀,出现空泡;浅层基质层胶原纤维排列疏松,空隙增大,提示基质水肿,角膜其余各层及晶状体未见损伤。结论初步探究出微瓦量级297 nm窄带LED光源功率密度5、10、15μW/cm^(2)单次照射幼兔角膜的大致损伤阈值分别为30、24、18 mJ/cm^(2)。发现不同功率密度损伤阈值不同,功率密度越低,损伤阈值越高,且损伤部位主要为角膜上皮层及基质浅层。Objective To investigate the damage threshold and pathological characteristics of cornea of infancy rabbits irradiated by 297 nm narrow-band LED at extremely low light intensity of microwatt.Methods The subjects of the study were 7 Chinese black rabbits with 14 eyes,irradiated by a narrow-band LED light source with a wavelength of(297±5)nm.According to different power densities,the rabbits were divided into 3 groups:5μW/cm^(2) group(Group A),10μW/cm^(2) group(group B),15μW/cm^(2) group(group C),and according to different energy density,each power density group was further divided into 4 subgroups:18 mJ/cm^(2) group(A1,B1,C1),24 mJ/cm^(2) group(A2,B2,C2),30 mJ/cm^(2) group(A3,B3,C3),36 mJ/cm^(2) group(A4,B4,C4).There were 2 eyes in the control group and 1 eye in each experimental group.Corneal turbidity was observed with slit lamp every 6 h after irradiation,and the site,area and depth of corneal damage were evaluated and recorded by fluorescein sodium staining 24 h after irradiation,which were the basis for determining the damage threshold.HE staining was performed to evaluate the pathological changes of corneal tissues.Results The damage thresholds of 297 nm narrow-band LED irradiation at power densities of 5,10 and 15μW/cm^(2) were 30,24 and18 mJ/cm^(2),respectively.The results of sodium fluorescein staining showed that punctate and flaky staining appeared in the corneal pupil area 24 h after exposure to the doses above the damage threshold and damage threshold.HE staining showed that corneal epithelial cells were sparsely distributed and disordered,and basal layer cells were swollen and vacuolated.The arrangement of superficial stromal collagen fibers was loose and the void increased,indicating stromal edema.No damage was found in other cornea layers and lens.Conclusions The approximate damage thresholds of cornea of 297 nm narrow-band LED irradiation at power densities of 5,10 and 15μW/cm^(2) were 30,24 and 18 mJ/cm^(2),respectively.The lower the power density,the higher the damage threshold,and the dam

关 键 词：窄带LED 极低强度 角膜 紫外线角膜炎 损伤阈值 

分 类 号：R318.51[医药卫生—生物医学工程] R772.21[医药卫生—基础医学]