干旱胁迫下高羊茅转录组学分析  

Transcriptome Analysis of Festuca elata under Drought Stress

作　　者：张沥曼玲 陈锡[2] 王小利[2] 陈功喜 昌西 ZHANG Limanling;CHEN Xi;WANG Xiaoli;CHEN Gongxi;CHANG Xi(Plant Science College,Xizang Agricultural and Animal Husbandry University,Linzhi Xizang 860000,China;Guizhou Institute of Prataculture,Guizhou Academy of Agricultural Sciences,Guiyang 550006,China)

机构地区：[1]西藏农牧学院植物科学学院,西藏林芝860000 [2]贵州省农业科学院草业研究所,贵阳550006

出　　处：《种子》2025年第2期69-77,共9页Seed

基　　金：贵州省科技计划项目(黔科合基础-ZK[2022]一般222);贵州省农业科学院青年科技基金项目(黔农科开放基金[2024]01)。

摘　　要：以高羊茅品种黔草1号叶片为材料,运用Illumina测序平台进行转录组测序分析,探究高羊茅在干旱胁迫下的相关基因和分子水平上的耐旱机制。结果表明,筛选出差异表达基因(DEGs)并作功能注释分析,获得7286个上调基因和7937个下调基因。GO功能注释后发现,DEGs主要在细胞核、核糖体和核糖体合成等过程中富集;KEGG富集分析发现,主要在核糖体生物合成、核糖体、植物激素信号转导、光合作用-天线蛋白等显著富集了DEGs。干旱胁迫下,与光合作用相关的捕光叶绿素a/叶绿素b结合蛋白基因上调,表明干旱会影响叶绿素对光的收集。而与植物激素相关的基因也被调控,生长素、细胞分裂素和脱落酸等通路都在响应,抵抗干旱所带来的胁迫影响。运用实时荧光定量PCR(qRT-PCR)技术验证,表明所选的差异表达基因与测序结果基本一致。The leaves of Festuca elata Qiancao No.1 were used as materials,and transcriptome sequencing was performed by Illumina sequencing platform to explore the drought tolerance mechanism of Festuca elata at the related gene and molecular level under drought stress.The results showed that 7286 up-regulated genes and 7937 down-regulated genes were obtained by differential expression genes(DEGs)and functional annotation analysis.After GO functional annotation,it was found that DEGs was mainly enriched during nuclear,ribosome and ribosome synthesis.KEGG enrichment analysis showed that DEGs was significantly enriched mainly in ribosome biosynthesis,ribosome,plant hormone signal transduction,photosynthesis-antenna protein,etc.Under drought stress,the light-harvesting chlorophyll a/chlorophyll b binding protein genes related to photosynthesis were up-regulated,indicating that drought would affect the collection of light by chlorophyll.The genes related to plant hormones were also regulated,and the growth hormone,cytokinin and abscisic acid pathways were responding to the stress effects of drought.Real-time quantitative PCR(qRT-PCR)proved that the selected differential expression genes were consistent with the sequencing results.

关 键 词：高羊茅 干旱胁迫 转录组分析 基因 

分 类 号：S688.4[农业科学—观赏园艺]