大豆SLAC/SLAH基因家族的鉴定与表达分析  

Identification and Expression Analysis of Glycine max SLAC/SLAH Gene Family

作　　者：孙云萌 段香波 刘冰 申星亮 杨帅 于洋 SUN Yunmeng;DUAN Xiangbo;LIU Bing;SHEN Xingliang;YANG Shuai;YU Yang(College of Life Science and Engineering,Shenyang University,Shenyang 110044,China;Liaoning Key Laboratory of Urban Integrated Pest Management and Ecological Security,Shenyang 110044,China)

机构地区：[1]沈阳大学生命科学与工程学院,沈阳110044 [2]辽宁省城市有害生物治理与生态安全重点实验室,沈阳110044

出　　处：《种子》2025年第2期78-86,94,共10页Seed

基　　金：国家自然科学基金青年科学基金项目(32201730);沈阳大学大学生创新创业训练计划项目(202311035235)。

摘　　要：慢阴离子通道(Slow anion channel,SLAC/SLAH)是植物中较保守的一个家族。在全基因组水平上对大豆SLAC/SLAH家族基因进行鉴定,通过生物信息学方法,对其理化性质、系统进化关系、共线性关系、二级结构、跨膜结构域、亚细胞定位、保守基序、基因结构以及顺式作用元件进行全面分析。结果表明,大豆基因组中含有11个SLAC/SLAH基因,可分为两个亚组,亚组内成员之间存在共线性关系,且保守基序和基因结构更为相似。大豆所有的SLAC/SLAH蛋白均含有数个跨膜结构域且定位于细胞膜。启动子分析显示,该家族基因含大量与激素和胁迫应答相关的顺式作用元件。对SLAC/SLAH家族基因的组织表达和盐胁迫下的表达模式进行分析,发现部分基因在大豆的根、种子或叶片中表达量比较高。经NaCl处理后,部分基因表达受到严重抑制,可能在大豆的盐胁迫应答过程中起重要作用。Slow anion channel(Slow anion channel,SLAC/SLAH)is a more conservative family in plants.The SLAC/SLAH family genes of Glycine max were identified at the whole genome level.The physicochemical properties,phylogenetic relationship,collinearity relationship,secondary structure,transmembrane domain,subcellular localization,conserved motif,gene structure and cis-acting elements were analyzed by bioinformatics methods.The results showed that the Glycine max genome contains 11 SLAC/SLAH genes,which could be divided into two subgroups,and there were collinear relationships among the members of the subgroups,and the conserved motif and gene structure were more similar.All Glycine max SLAC/SLAH proteins contained several transmembrane domains and were localized to the cell membrane.Promoter analysis revealed that this family of genes contained a large number of cis-acting elements related to hormone and stress response.In addition,the study also analyzed the tissue expression and expression patterns of this family gene under salt stress,and found that some genes were highly expressed in the roots,seeds or leaves of Glycine max.After NaCl treatment,the expression of some genes was severely inhibited,which might play an important role in the salt stress response of Glycine max.

关 键 词：大豆 慢阴离子通道 生物信息学分析 基因表达 

分 类 号：S565.1[农业科学—作物学]