基于网络药理学探讨柴胡皂苷a的抗抑郁作用机制  

Mechanism of anti-depression action of saikosaponin a based on network pharmacology

作　　者：司玉芳 刘品 王平 李午戊[1] SI Yufang;LIU Pin;WANG Ping;LI Wuwu(Department of Life Sciences,College of Chemistry and Chemical Engineering,Xianyang Normal University,Xianyang 712000,China;Engineering Research Center for Loess PlateauTraditional Chinese Medicine,Universities of Shaanxi Province,Xianyang Normal University,Xianyang 712000,China)

机构地区：[1]咸阳师范学院化学与化工学院生命科学系,陕西咸阳712000 [2]咸阳师范学院黄土高原道地药材陕西省高校工程研究中心,陕西咸阳712000

出　　处：《现代药物与临床》2025年第2期269-276,共8页Drugs & Clinic

基　　金：陕西省自然科学基础研究计划(2024JC-YBMS-119,2021JQ-816);陕西省教育厅重点科学研究计划项目(高校工程研究中心项目)(24JR162);陕西基础科学(化学、生物学)研究院基础科学研究计划(22JHQ050)。

摘　　要：目的通过网络药理学探讨柴胡皂苷a在抑郁症治疗中的作用机制。方法从PubChem、PharmMapper和SwissTarget Prediction数据库中获取柴胡皂苷a的分子结构和潜在靶点。将柴胡皂苷a和抑郁症的共同靶点导入STRING 11.0数据库,构建蛋白相互作用(PPI)网络。利用DAVID 6.8数据库进行基因本体论(GO)和京都基因与基因组百科全书(KEGG)富集分析。结果本研究共获得柴胡皂苷a靶点408个,抑郁相关靶点3799个,最终得到200个共同靶点,包括白蛋白(ALB)、丝氨酸/苏氨酸蛋白激酶B(Akt1)、热休克蛋白90α家族A类成员1(HSP90AA1)、转录信号转导和激活因子3(STAT3)和表皮生长因子受体(EGFR)等。GO和KEGG富集分析表明,癌症、脂质和动脉粥样硬化、前列腺癌、癌症蛋白多糖、内分泌耐药、叉头框-O(FoxO)信号通路、磷酸肌醇-3激酶(PI3K)/Akt信号通路、EGFR酪氨酸激酶抑制剂耐药、Th17细胞分化、催乳素信号通路等通路在柴胡皂苷a抗抑郁中发挥了重要作用。结论柴胡皂苷a可能通过作用于FoxO、催乳素、PI3K/Akt信号通路及ALB、Akt1、HSP90AA1、STAT3等靶点发挥抗抑郁作用。Objective To elucidate the mechanism of saikosaponin a in treating depression with network pharmacology.Methods Molecular structure and potential targets of saikosaponin a was obtained from PubChem,PharmMapper and SwissTarget Prediction databases.The common targets of saikosaponin a and depression were imported to the STRING 11.0 database,and then a protein interaction network was constructed.GO and KEGG enrichment were analyzed with DAVID 6.8 database.Results A total of 408 targets of saikosaponin a were obtained and there were 3799 depression-related targets.A total of 200 common genes were selected as targets of depression including ALB,Akt1,HSP90AA1,STAT3,and EGFR.GO and KEGG analysis suggested that pathways in cancer,lipid and atherosclerosis,prostate cancer,proteoglycans in cancer,endocrine resistance,FoxO signaling pathway,PI3K/Akt signaling pathway,EGFR tyrosine kinase inhibitor resistance,Th17 cell differentiation,and prolactin signaling pathway played an imported role.Conclusion Our results suggested that saikosaponin a may exert its antidepressant effect by acting on FoxO,prolactin,and PI3K/Akt signaling pathways and ALB,Akt1,HSP90AA1,and STAT3 targets.

关 键 词：柴胡皂苷A 抑郁症 网络药理学 白蛋白 热休克蛋白90α家族A类成员1 叉头框-O信号通路 磷酸肌醇-3激酶/蛋白激酶B 

分 类 号：R285[医药卫生—中药学] R286.1[医药卫生—中医学]