多指标成分定量联合化学计量学评价不同产地独脚金质量  

Quality evaluation of Striga asiatica from different producing areas by multiindex component quantification combined with chemometrics

作　　者：曹勇 陆以云[3] 杨波 CAO Yong;LU Yiyun;YANG Bo(Department of Pharmacy,Beijing Friendship Hospital,Capital MedicalUniversity,Beijing 100050,China;The Second Clinical Medical College of Capital Medical University,Beijing 100050,China;Institute of Traditional Chinese Medicine National Drug Testing,National Institutes for Food and Drug Control,Beijing 100050,China)

机构地区：[1]首都医科大学附属北京友谊医院药剂科,北京100050 [2]首都医科大学第二临床医学院,北京100050 [3]中国食品药品检定研究院中药民族药检定所,北京100050

出　　处：《现代药物与临床》2025年第2期341-348,共8页Drugs & Clinic

基　　金：国家中医药管理局全国中药特色技术传承人才培训项目[国中医药人教函(2023)96号]。

摘　　要：目的采用化学计量学、加权TOPSIS与灰色关联度融合模型评价不同产地独脚金的质量差异。方法采用HPLC法测定独脚金中木犀草素、芹菜素、金圣草素、香叶木素、金合欢素、毛蕊花糖苷、异毛蕊花糖苷、桃叶珊瑚苷、β-谷甾醇,同时检测水溶性浸出物、醇溶性浸出物、总灰分和酸不溶性灰分,对结果进行PCA和OPLS-DA分析,挖掘影响产品质量的差异性标志物。建立加权TOPSIS与GRA融合模型,对不同产地独脚金质量进行综合评价。结果18批独脚金批次间差异较大,PCA结果显示18批独脚金聚为3类,OPLS-DA分析从13个变量中筛选出4个差异性标志物。加权TOPSIS与灰色关联度融合模型显示18批样品的综合相对贴近度在0.2931~0.6658,以S14综合相对贴近度最高。结论所建方法分析用时短,结果准确,可用于独脚金多指标成分定量控制和质量评价。Objective To evaluate the quality difference of Striga asiatica from different producing areas by chemometrics,weighted TOPSIS,and grey correlation degree fusion model.Methods The contents of luteolin,apigenin,chrysoeriol,diosmetin,acacetin,verbascoside,isoacteoside,aucubin andβ-sitosterol in S.asiatica were simultaneously determined by HPLC.At the same time,the water-soluble extract,alcohol-soluble extract,total ash,and acid-insoluble ash were detected.PCA and OPLS-DA were used to analyze the quantitative detection results,and the differential markers affecting product quality were excavated.A weighted TOPSIS and GRA fusion model was established to comprehensively evaluate the quality of S.asiatica from different producing areas.Results There was a large difference among 18 batches of S.asiatica.PCA results showed that S.asiatica were clustered into 3 categories.OPLSDA analysis screened 4 differential markers from 13 variables.The fusion model of weighted TOPSIS and grey correlation degree showed that the comprehensive relative closeness of 18 batches of samples was between 0.2931 and 0.6658,and the comprehensive relative closeness of S14 was the highest.Conclusion The established method is convenient and accurate,and can be used for quantitative control and quality evaluation for S.asiatica.

关 键 词：独脚金 木犀草素 芹菜素 高效液相色谱 PCA OPLS-DA 加权TOPSIS 灰色关联度分析法 

分 类 号：R286.02[医药卫生—中药学]