电针预处理对脑缺血再灌注大鼠行为学及外周血内皮祖细胞的影响  

Effects of electroacupuncture preconditioning on behavioural and peripheral blood EPCs in cerebral ischaemia-reperfusion injury rats

作　　者：张艺 任秀君[1] 郭孟玮[1] 赵雅芳[1] 李一凡 李佳阳 任晓暄[1] 邬继红[1] 卢海洋 Yi Zhang;Xiujun Ren;Mengwei Guo;Yafang Zhao;Yifan Li;Jiayang Li;Xiaoxuan Ren;Jihong Wu;Haiyang Lu(School of Acupuncture-Moxibustion and Tuina,Beijing University of Traditional Chinese Medicine,Beijing 102488,China;Department of Anesthesiology,Beijing Friendship Hospital,Capital Medical University,Beijing 100050,China)

机构地区：[1]北京中医药大学针灸推拿学院,北京102488 [2]首都医科大学附属北京友谊医院麻醉科,北京100050

出　　处：《中华神经创伤外科电子杂志》2024年第2期71-77,共7页Chinese Journal Of Neurotraumatic Surgery:Electronic Edition

摘　　要：目的观察电针预处理对脑缺血再灌注损伤(CIRI)大鼠行为学及外周血内皮祖细胞(EPCs)水平的影响。方法45只成年雄性SD大鼠随机分为假手术组、模型组和电针预处理组(EA组),每组15只。模型组于造模前将大鼠束缚14 d,采用Longa线栓法制备大脑中动脉缺血再灌注模型;假手术组除不插入线栓外,余同模型组;EA组在造模前14 d行电针预处理,余同模型组。造模当天监测大鼠脑血流量,造模后1 d计算脑梗死体积,造模后1、3、7、21、28 d分别采用改良神经功能缺损评分(mNSS)、旷场实验和Morris水迷宫实验对大鼠相关指标进行检测,取外周血采用流式细胞术测定EPCs水平。结果模型组和EA组造模过程中各时间点的脑血流量明显低于假手术组,EA组在线栓即刻及再灌注30 min的脑血流量高于模型组,差异均有统计学意义(P<0.05)。模型组和EA组脑梗死体积百分比显著高于假手术组,EA组脑梗死体积百分比显著低于模型组,差异均有统计学意义(P<0.05)。EA组造模后1、3、7及21 d的mNSS评分均低于模型组,差异有统计学意义(P<0.05)。EA组大鼠旷场实验的平均速度在造模后1 d明显高于模型组,目标象限滞留时间百分比在造模后3 d高于模型组,差异有统计学意义(P<0.05)。外周血EPCs水平在造模后2 d高于模型组,差异均有统计学意义(P<0.05)。结论电针预处理一定程度上可以改善急性期CIRI大鼠的脑血流状态,减轻神经功能损伤,提升学习记忆能力和运动能力,提高外周血中EPCs水平。Objective To observe the effects of electroacupuncture pretreatment on behavioral and peripheral blood endothelial progenitor cells(EPCs)levels in rats with cerebral ischemia-reperfusion injury(CIRI).Methods Forty-five adult male SD rats were randomly divided into the sham group,the model group and the electroacupuncture pretreatment group(EA group),with 15 rats in each group.Rats in the model group were restrained for 14 d before surgery,and the middle cerebral artery occlusion ischemia-reperfusion model was established by Longa thread-occluded method;rats in the sham group were treated as the same as the rats in the model group except for not inserting thread plug;rats in the EA group were pretreated with EA 14 d before surgery,others were the same as those in the model group.Cerebral blood flow was monitored on the day of modelling,and infarct volume was calculated 1 d after modelling.At different time points after modelling,the modified neurological severity score(mNSS),the open-field experiment and the Morris water maze experiment were used to detect the relevant indexes in rats,and peripheral blood was taken to determine the levels of EPCs by flow cytometry.Results The cerebral blood flow at each time point during the modeling process in the model group and EA group was significantly lower than that in the sham surgery group.The cerebral blood flow in the EA group was higher than that in the model group immediately after thrombus insertion and 30 min of reperfusion,and the differences were statistically significant(P<0.05).The cerebral infarction volume percentage in the model group and EA group was significantly higher than that in the sham surgery group,while the cerebral infarction volume percentage in the EA group was significantly lower than that in the model group,with statistical significance(P<0.05).The mNSS scores of the EA group at 1,3,7,and 21 d after modeling were lower than those of the model group,and the difference was statistically significant(P<0.05).The average speed of the open field expe

关 键 词：电针预处理 脑缺血再灌注损伤 大脑中动脉栓塞 内皮祖细胞 

分 类 号：R245[医药卫生—针灸推拿学]