囊胚二次玻璃化冷冻对临床妊娠结局的影响  

Effect of secondary vitrification freezing of blastocyst on clinical pregnancy outcome

作　　者：莫房德 施丽 牛向丽 公方强 MO Fangde;SHI Li;NIU Xiangli;GONG Fangqiang(Department of Reproduction,The Reproductive Hospital of Guangxi Zhuang Autonmous Region,Nanning 530022,P.R.China)

机构地区：[1]广西壮族自治区生殖医院生殖科,南宁530022

出　　处：《中国妇产科临床杂志》2025年第2期121-124,共4页Chinese Journal of Clinical Obstetrics and Gynecology

基　　金：广西壮族自治区生殖医院自筹经费课题(SZYY2021265)。

摘　　要：目的探讨囊胚二次玻璃化冷冻对临床妊娠结局的影响。方法回顾性分析2018年8月至2023年12月进行二次玻璃化冷冻单囊胚移植57例患者的临床资料,共57个周期纳入二次冷冻组。并从同期的常规单次玻璃化冷冻复苏单囊胚移植周期1148例中简单随机选取153例作为单次冷冻组,与二次冷冻组基本资料匹配。比较两组患者的一般资料、临床妊娠率、种植率、流产率、异位妊娠率等方面。同时比较二次冷冻与单次冷冻移植不同时期(D5囊胚与D6囊胚)的单囊胚以及不同评级(优质囊胚与非优质囊胚)的单囊胚的临床妊娠率、种植率、流产率、异位妊娠率等。结果二次冷冻组临床妊娠率(33.92%,19/56)与单次冷冻组(52.28%,80/153)比较,差异有统计学意义(P=0.01)。移植D5囊胚的二次冷冻组临床妊娠率(36.11%,13/36)与单次冷冻组(55.75%,6/20)比较,差异有统计学意义(P<0.05)。移植D6囊胚的二次冷冻组临床妊娠率(30%,13/36)与单次冷冻组(42.5%,17/40)比较,差异无统计学意义(P>0.05)。移植优质囊胚的二次冷冻组临床妊娠率(39.28%,11/28)与单次冷冻组(59.18%,58/98)比较,差异无统计学意义(P>0.05)。移植非优质囊胚的二次冷冻组临床妊娠率(28.57%,8/28)与单次冷冻组(40%,22/55)比较,差异无统计学意义(P>0.05)。两组患者的流产率、异位妊娠率,差异无统计学意义(P>0.05),两组患者移植不同时期以及不同评级单囊胚的流产率、异位妊娠率,差异无统计学意义(P>0.05)。结论二次冷冻会降低临床妊娠率,反复冻融可能影响胚胎发育潜能,临床工作中尽量避免反复冻融。Objective To investigate the effect of secondary vitrification freezing of blastocyst on clinical pregnancy outcomes.Methods The clinical data of 57 patients who underwent secondary vitrification frozen single blastocyst transplantation from August 2018 to December 2023 were retrospectively analyzed,and a total of 57 cycles were included in the secondary freezing group.In addition,153 cases were randomly selected as the single freezing group from 1148 cases of single vitrification cryopreservation during the same period,which matched the basic data of the secondary freezing group.The differences in general data,clinical pregnancy rate,implantation rate,abortion rate and ectopic pregnancy rate of two groups were compared.At the same time,the clinical pregnancy rate,implantation rate,abortion rate and ectopic pregnancy rate of the single blastocysts at different periods(D5 blastocysts and D6 blastocysts)and the single blastocysts with different ratings(high quality blastocysts and non-high quality blastocysts)were compared.Results The clinical pregnancy rate of the secondary freezing group(33.92%,19/56)was significantly lower than that of the single freezing group(52.28%,80/153)(P=0.01).The clinical pregnancy rate of D5 blastocyst transplantation in the secondary freezing group(36.11%,13/36)was significantly lower than that of the single freezing group(55.75%,6/20)(P<0.05).There was no significant difference in the clinical pregnancy rate of D6 blastocyst transplantation between the secondary freezing group(30%,13/36)and the single freezing group(42.5%,17/40)(P>0.05).There was no significant difference in clinical pregnancy rate of high quality blastocysts between the secondary freezing group(39.28%,11/28)and the single freezing group(59.18%,58/98)(P>0.05).There was no significant difference in the clinical pregnancy rate of non-high quality blastocysts between the secondary freezing group(28.57%,8/28)and the single freezing group(40%,22/55)(P>0.05).There was no significant difference in the abortion rate and ectop

关 键 词：玻璃化冷冻 二次冷冻 临床妊娠结局 单囊胚移植 

分 类 号：R714.8[医药卫生—妇产科学]