负载纳米二硫化钼丝素蛋白-壳聚糖复合膜的抗菌活性及其在烫伤治疗中的应用  

Antimicrobial Activity of Molybdenum Disulfide-loaded Nanofilament Protein-chitosan Composite Membrane and Its Application in the Treatment of Burns

作　　者：王大俊 陶玉贵[1] 金伟豪 梁杰 WANG Dajun;TAO Yugui;JIN Weihao;LIANG Jie(School of Biological and Food Engineering,Anhui Polytechnic University,Wuhu 241000,China)

机构地区：[1]安徽工程大学生物与食品工程学院,安徽芜湖241000

出　　处：《安徽工程大学学报》2025年第1期44-52,共9页Journal of Anhui Polytechnic University

基　　金：安徽省自然科学研究重点项目(KJ2021A1288)。

摘　　要：本研究成功制备了负载绿原酸(CGA)修饰的二硫化钼(MoS_(2))纳米颗粒,并利用这些纳米颗粒开发出一种新型的多功能丝素蛋白(SF)-壳聚糖(CS)复合膜,旨在评估其在物理特性、抗菌活性以及促进烫伤伤口愈合方面的潜力。采用水热法合成MoS_(2)纳米颗粒,并通过CGA对其表面进行修饰,以增强其与CS和SF的相容性和交联能力。随后,通过交联形成MoS_(2)@CGA-CS/SF(MCCS)复合膜,经过一系列的物理和生物学评价,展现了其优异的性能。基础表征结果表明,MoS_(2)纳米颗粒在MCCS复合膜中呈均匀分布,且具有约150~200 nm的一致直径。通过比较发现,以9:1的比例混合交联得到的复合膜,在透气性和抗张强度方面显著优于单一的CS或SF膜。此外,MCCS复合膜的光热转换效率高达36.6%,在抗菌性能测试中,对耐药性大肠杆菌和金黄色葡萄球菌的抑制率均超过90%。利用小鼠烫伤伤口模型进行的体内实验进一步证实,MCCS复合膜不仅具有卓越的物理性能和抗菌活性,而且在促进烫伤伤口愈合方面表现出极大的潜力,证明了其在临床应用中的前景。Chlorogenic acid(CGA)-modified molybdenum disulfide(MoS_(2))nanoparticles were successfully synthesized and utilized these nanoparticles to develop a novel multifunctional silk fibroin(SF)-chitosan(CS)composite membrane.The aim was to evaluate its potential in terms of physical properties,antimicrobial activity,and promoting the healing of scald wounds.The MoS_(2) nanoparticles were synthesized by using a hydrothermal method and surface-modified with CGA to enhance their compatibility and crosslinking ability with chitosan and silk fibroin.Subsequently,the MoS_(2)@CGA-CS/SF(MCCS)composite membrane formed through crosslinking underwent a series of physical and biological evaluations,demonstrating its superior performance.Basic characterization results indicated that MoS_(2) nanoparticles were uniformly distributed within the MCCS composite membrane,exhibiting a consistent diameter of approximately 150~200 nm.Comparatively,the composite membrane prepared by mixing and crosslinking at a ratio of 9:1 exhibited significantly superior air permeability and tensile strength compared to the individual CS or SF membranes.Furthermore,the MCCS composite membrane displayed a high photothermal conversion efficiency of 36.6% and,in antimicrobial performance tests,inhibited resistant Escherichia coli and Staphylococcus aureus with inhibition rates above 90%.Vivo experiments utilizing a mouse scald wound model further confirmed the efficacy of the MCCS composite membrane in promoting wound healing.After 12 days of treatment,the scald wounds in mice were almost completely healed,demonstrating the significant role of the MCCS composite membrane in promoting the healing of scald wounds.In conclusion,the MCCS composite membrane not only exhibited outstanding physical properties and antimicrobial activity but also demonstrated great potential in promoting the healing of scald wounds,thereby proving its prospects for clinical application.

关 键 词：丝素蛋白-壳聚糖复合膜 二硫化钼纳米球 光热疗法 抗菌活性 烫伤敷料 

分 类 号：R318.08[医药卫生—生物医学工程] TB332[医药卫生—基础医学]