Ischemia-reperfusion injury after spinal cord decompressive surgery-An in vivo rat model  

作　　者：Boyu Zhang Zhefeng Jin Pengren Luo He Yin Xin Chen Bowen Yang Xiaokuan Qin LiGuo Zhu Bo Xu Guoliang Ma Dian Zhang 

机构地区：[1]Sports medicine department 3,Wangjing Hospital Affiliated to China Academy of Chinese Medical Sciences,Beijing,China [2]Spine Department 2,Wangjing Hospital Affiliated to China Academy of Chinese Medical Sciences,Beijing,China

出　　处：《Animal Models and Experimental Medicine》2025年第3期405-420,共16页动物模型与实验医学(英文)

基　　金：Wangjing Hospital Affiliated to China Academy of Chinese Medical Sciences,Grant/Award Number:WJYY-YJKT-2022-13。

摘　　要：Background:Although decompression surgery is the optimal treatment for patients with severe degenerative cervical myelopathy(DCM),some individuals experience no improvement or even a decline in neurological function after surgery,with spinal cord ischemia–reperfusion injury(SCII)identified as the primary cause.Spinal cord compression results in local ischemia and blood perfusion following decompression is fundamental to SCII.However,owing to inadequate perioperative blood flow monitoring,direct evidence regarding the occurrence of SCII after decompression is lacking.The objective of this study was to establish a suitable animal model for investigating the underlying mechanism of spinal cord ischemia–reperfusion injury following decom-pression surgery for degenerative cervical myelopathy(DCM)and to elucidate alterations in neurological function and local blood flow within the spinal cord before and after decompression.Methods:Twenty-four Sprague–Dawley rats were allocated to three groups:the DCM group(cervical compression group,with implanted compression material in the spinal canal,n=8),the DCM-D group(cervical decompression group,with removal of compression material from the spinal canal 4 weeks after implantation,n=8),and the SHAM group(sham operation,n=8).Von Frey test,forepaw grip strength,and gait were assessed within 4 weeks post-implantation.Spinal cord compression was evaluated using magnetic resonance imaging.Local blood flow in the spinal cord was monitored during the perioperative decompression.The rats were sacrificed 1 week after decompression to observe morphological changes in the compressed or decompressed segments of the spinal cord.Additionally,NeuN expression and the oxidative damage marker 8-oxoG DNA were analyzed.Results:Following spinal cord compression,abnormal mechanical pain worsened,and a decrease in forepaw grip strength was observed within 1–4 weeks.Upon decompression,the abnormal mechanical pain subsided,and forepaw grip strength was restored;however,neither reached the level

关 键 词：8-oxoG DNA degenerative cervical myelopathy spinal cord ischemia-reperfusion injury surgical decompression 

分 类 号：R54[医药卫生—心血管疾病]