Effectively simplified Adriamycin-induced chronic kidney disease mouse model:Retro-orbital vein injection versus tail-vein injection  

作　　者：Masaki Watanabe Hayato R.Takimoto Kazuki Hashimoto Yuki Ishii Nobuya Sasaki 

机构地区：[1]Laboratory of Laboratory Animal Science and Medicine,School of Veterinary Medicine,Kitasato University,Towada,Japan

出　　处：《Animal Models and Experimental Medicine》2025年第3期568-572,共5页动物模型与实验医学(英文)

摘　　要：This study aimed to investigate the impact of administration routes in establishing the Adriamycin(ADR)-induced chronic kidney disease(CKD)model.Using BALB/c mice,we compared the effects of conventional tail-vein injection(TV10,10 mg/kg)to those of retro-orbital sinus(orbital vein)injection(OV10,10 mg/kg;OV8,8 mg/kg).The re-sults indicated that the OV10 group exhibited CKD pathology similar to the TV10 group,with both groups demonstrating significantly higher urinary albumin/creatinine ratio(p<0.05),tubular injury(p<0.05),and degree of renal fibrosis(p<0.05)than the OV8 group.No significant differences were observed between the OV10 and TV10 groups in urinary albumin/creatinine ratio,tubular injury,and degree of renal fibrosis.These findings demonstrated that retro-orbital administration of 10 mg/kg ADR in-duces comparable effects to conventional tail-vein administration.This technique's technical simplicity may improve experimental efficiency,reproducibility,and animal welfare in CKD research.In conclusion,this study validates the utility of retro-orbital injection in CKD model establishment,demonstrating its potential to standardize and improve the reliability of future CKD research protocols.

关 键 词：Adriamycin nephropathy BALB/c CKD orbital vein injection tail-vein injection 

分 类 号：R69[医药卫生—泌尿科学]