基于二维斑点追踪技术的纵向分层应变评价达格列净干预早期自发性高血压大鼠心肌功能变化  

Effect of dapagliflozin on myocardial function changes in early spontaneously hypertensive rats:a study based on longitudinal layer-specific strain

作　　者：陈雪梅 张君 李欣 姚冉 刘灯 冯江 谭琳 郭燕丽 CHEN Xuemei;ZHANG Jun;LI Xin;YAO Ran;LIU Deng;FENG Jiang;TAN Lin;GUO Yanli(Department of Ultrasonography,First Affiliated Hospital,Army Medical University(Third Military Medical University),Chongqing,China)

机构地区：[1]陆军军医大学(第三军医大学)第一附属医院超声科,重庆

出　　处：《陆军军医大学学报》2025年第6期531-538,共8页Journal of Army Medical University

基　　金：重庆英才创新领军人才项目(425Z2K1);重庆市技术创新与应用发展专项重点项目(CSTB2022TIAD-KPX0153)。

摘　　要：目的基于二维斑点追踪技术的纵向分层应变参数评估达格列净对早期自发性高血压大鼠(spontaneously hypertensive rat,SHR)心肌功能的作用。方法将45只6周龄大小的雄性SHR随机分为3组(n=15):对照组、达格列净组以及氯沙坦组,达格列净组、氯沙坦组分别以达格列净1 mg/kg、氯沙坦10 mg/kg每天给药喂养连续8周,对照组以等量的生理盐水喂养;同周龄的正常血压雄性Wistar京都大鼠(wistar-kyoto rat,WKY)15只作为空白对照组(WKY组)。每2周对各组行尾动脉收缩压测量、常规超声心动图和二维斑点追踪图像采集及分析,获取左心室心肌各层纵向应变,比较各组参数差异,观察大鼠心肌病理改变。结果与WKY组相比,第8周时,对照组左室收缩功能参数左室射血分数(left ventricular ejection fraction,LVEF)、左室短轴缩短率(left ventricular fraction shortening,LVFS)均下降(P<0.05),而达格列净组及氯沙坦组LVEF、LVFS无明显下降。与WKY组相比,在第6周时对照组的心内膜下心肌整体纵向应变(global longitudinal strain of endo-myocardium,GLSendo)已出现下降(P<0.05);第8周时,对照组的GLSendo、中层心肌整体纵向应变(global longitudinal strain of mid-myocardium,GLSmid)及心外膜下心肌整体纵向应变(global longitudinal strain of epi-myocardium,GLSepi)均下降(P<0.05);而达格列净组、氯沙坦组的纵向分层应变参数较WKY组均无统计学差异(P>0.05)。病理结果进一步证实,实验第6周时,对照组已出现心肌间质纤维化。结论达格列净可有效改善早期SHR的心肌功能。Objective To evaluate the effect of dapagliflozin on myocardial function in early spontaneously hypertensive rats(SHR)with layer-specific global longitudinal strain(GLS).Methods A total of 45 male SHR aged 6 weeks were randomly divided into control group(normal saline),dapagliflozin group[1 mg/(kg·day)],and losartan group[10 mg/(kg·day)].Fifteen male Wistar-Kyoto(WKY)rats at same age with normal blood pressure were subjected and served as blank control group.During 8 weeks of intervention,systolic blood pressure(SBP)was measured,and conventional echocardiography and two-dimensional speckle tracking echocardiography(2DSTE)were performed and the results were collected to acquire the longitudinal strain of each layer of left ventricular(LV)myocardium.The parameters were compared among the groups.The pathological changes of myocardium were observed in each group of rats.Results Compared with the WKY group,LV ejection fraction(LVEF)and LV fraction shortening(LVFS)at week 8 were decreased in the control group(P<0.05),but no such decreases were observed in the dapagliflozin group and the losartan group.The GLS of endo-myocardium(GLSendo)at the 6th week was decreased,and GLSendo,GLSmid and GLSepi at the 8th week were all decreased in the control group than the WKY group(all P<0.05).But there were no statistical differences in the above 3 indicators in the dapagliflozin and losartan groups when compared with the WKY group(all P>0.05).The pathological results showed that myocardial interstitial fibrosis was observed in the control group at the 6th week.Conclusion Dapagliflozin can effectively improve myocardial function in early SHR.

关 键 词：高血压 超声心动描记术 斑点追踪 左心室功能 达格列净 

分 类 号：R445.1[医药卫生—影像医学与核医学] R554.105[医药卫生—诊断学] R997.15[医药卫生—临床医学]