Rab37基因多态性与月经初潮延迟的关联研究  

Association of Rab37 gene polymorphism and delayed menarche:a report of 2060 women

作　　者：袁梦 蔡漾 房晓渝 吕宏尧 王娇[1] 马翔宇[1] YUAN Meng;CAI Yang;FANG Xiaoyu;LYU Hongyao;WANG Jiao;MA Xiangyu(Department of Epidemiology,Faculty of Military Preventive Medicine,Army Medical University(Third Military Medical University),Chongqing;Department of Disease Control and Prevention,General Hospital of Western Theater Command,Chengdu,Sichuan;College of Public Health,Southwest Medical University,Luzhou,Sichuan,China)

机构地区：[1]陆军军医大学(第三军医大学)军事预防医学系军队流行病学教研室,重庆 [2]西部战区总医院疾病预防控制科,四川成都 [3]西南医科大学公共卫生学院,四川泸州

出　　处：《陆军军医大学学报》2025年第6期623-628,F0003,共7页Journal of Army Medical University

基　　金：重庆英才青年拔尖人才基金(CQYC202005003)。

摘　　要：目的探究Rab37基因的单核苷酸多态性(single nucleotide polymorphism,SNP)与月经初潮年龄(age at menarche,AAM)的关联。方法本研究采用病例对照研究设计,对江苏省句容市某社区的2060名女性进行流行病学调查,并采集静脉血样。使用ASA芯片对血样DNA进行Rab37基因分型,获得参与者的SNP rs62084865、rs10512597、rs35489971、rs1037170、rs6501732、rs77822106、rs3178300分型结果。根据月经初潮时间<16周岁和≥16周岁将参与者分成2组。通过线性回归分析和Logistic回归分析,采用显性模型、隐性模型和等位基因模型,探索Rab37基因的多态性与月经初潮时间的关联性。结果在2060名健康女性中,944名女性月经时间正常,1116名女性出现月经初潮延迟。线性回归分析结果显示rs3178300的基因多态性与女性月经初潮时间呈负相关[隐性模型:CC/CT+TT,β=-0.915,95%CI(-1.692,-0.137),P=0.021;等位基因模型:T/C,β=-0.221,95%CI(-0.410,-0.032),P=0.022]。Logistic回归分析结果显示rs3178300的基因多态性与女性月经初潮推迟风险显著相关[隐性模型:CC/CT+TT,OR=0.295,95%CI(0.116,0.751),P=0.010;等位基因模型:T/C,OR=0.796,95%CI(0.652,0.972),P=0.025]。其余的6个SNP与月经初潮年龄的关联不显著。结论Rab37基因的rs3178300多态性与中国女性的月经初潮延迟显著相关。Objective To explore the association between single nucleotide polymorphism(SNP)of Rab37 gene and the age at menarche(AAM).Methods A case-control study design was used to conduct an epidemiologic survey on 2060 women in a community in Jurong City,Jiangsu Province,and their venous blood samples were collected.ASA chip was used to explore the Rab37 gene genotyping of blood sample DNA to obtain the genotyping results for Rab37 SNPs rs62084865,rs10512597,rs35489971,rs1037170,rs6501732,rs77822106,and rs3178300.Based on menarche time of<16 years old or≥16 years old,the participants were divided into 2 groups.Linear and logistic regression analyses were used to explore the association between the SNP of Rab37 gene and the age at menarche based on dominant,recessive and allelic models.Results Among the 2060 healthy women,944 women had normal menstrual periods,while 1116 women experienced delayed menarche.Linear regression analysis showed that the gene polymorphism of rs3178300 was negatively correlated with the age at menarche in females[recessive model:CC/CT+TT,β=-0.915,95%CI(-1.692,-0.137),P=0.021;allelic model:T/C,β=-0.221,95%CI(-0.410,-0.032),P=0.022].Logistic regression analysis indicated that the gene polymorphism of rs3178300 was significantly associated with the risk of delayed menarche in females[recessive model:CC/CT+TT,OR=0.295,95%CI(0.116,0.751),P=0.010;allelic model:T/C,OR=0.796,95%CI(0.652,0.972),P=0.025].The remaining 6 SNPs showed no significant association with age at menarche.Conclusion The rs3178300 polymorphism of Rab37 gene is significantly associated with delayed menarche in Chinese women.

关 键 词：月经初潮 单核苷酸多态性 病例对照研究 候选基因研究 

分 类 号：R181.33[医药卫生—流行病学] R446.9[医药卫生—公共卫生与预防医学] R711.5