银杏叶提取物及注射液中黄酮类成分分析与含量测定  

Component Analysis and Content Determination of Characteristic Flavonoids in Ginkgo biloba Extract and Its Injections

作　　者：胡庆丰 冉庆念 刘婕 王敬赫 惠爱玲 张文成 HU Qingfeng;RAN Qingnian;LIU Jie;WANG Jinghe;HUI Ailing;ZHANG Wencheng(School of Food and Biological Engineering,Hefei University of Technology,Hefei Anhui 230601,China;Engineering Research Center of Bio-Process of Ministry of Education(Hefei University of Technology),Hefei Anhui 230601,China)

机构地区：[1]合肥工业大学食品与生物工程学院,安徽合肥230601 [2]农产品生物化工教育部工程研究中心(合肥工业大学),安徽合肥230601

出　　处：《广西师范大学学报(自然科学版)》2025年第2期238-246,共9页Journal of Guangxi Normal University:Natural Science Edition

基　　金：江苏省苏北科技专项(XZ-SZ202030)。

摘　　要：为研究银杏叶提取物(GBE)及其注射液中特征性黄酮类成分,本文采用HPLC指纹图谱、液质联用等方法对其中共有且主要的黄酮类成分进行分析,并建立HPLC定量检测方法对黄酮含量进行测定。结果表明:GBE及其注射液中芦丁、异槲皮苷、山柰酚-3-O-芸香糖苷、水仙苷、紫云英苷、槲皮素、山柰酚、异鼠李素等8种成分为GBE含量较高且具特征性的黄酮成分;采用C 18色谱柱,乙腈-0.2%磷酸梯度洗脱,流速1.0 mL/min,检测波长360 nm,柱温30℃进行HPLC定量检测,8种黄酮成分分别在6.25~75.00、1.00~12.00、1.75~21.00、1.75~21.00、1.25~15.00、1.25~15.00、0.50~6.00和0.50~6.00 mg/L范围内线性关系良好(r>0.999),其精密度、重复性、稳定性及加样回收率RSD均小于2%;3种GBE粉末中8种黄酮总含量分别为61.37、76.23、140.55 mg/g,4种GBE注射液的黄酮总含量分别为246.0、305.2、284.5和250.8 mg/L。该研究为高品质GBE原料药及其注射液制品的质量控制提供科学依据。To analyze the characteristic flavonoids in Ginkgo biloba extract(GBE)and its injections,the HPLC fingerprint and liquid chromatography-mass spectrometry(LC-MS)were used to determine the flavonoid components.Additionally,a HPLC quantitative detection method for determining the total flavonoids content was established.Eight flavonoids including rutin,isoquercetin,kaempferol-3-O-rutinoside,narcissus,astragalin,quercetin,kaempferol and isorhamnetin occupied the high content in GBE and injections,which served as the characteristic flavonoid components for further research.The HPLC quantitative detection was performed using a C18 column,acetonitrile-0.2%phosphoric acid as gradient elution,flow rate of 1.0 mL/min,detection wavelength of 360 nm,and column temperature of 30℃.Eight flavonoids demonstrated good linear relationship(r>0.999)when their concentration ranged from 6.25 to 75.00,1.00 to 12.00,1.75 to 21.00,1.75 to 21.00,1.25 to 15.00,1.25 to 15.00,0.50 to 6.00 and 0.50 to 6.00 mg/L,respectively.The precision,repeatability,stability,and recovery rate RSD were less than 2%.The total flavonoid content in three GBEs were 61.37,76.23,140.55 mg/g,respectively.Also,four injections gave the results of 246.0,305.2,284.5,250.8 mg/L.This study identifies eight characteristic flavonoids in GBE and its injections and establishes a quantitative detection method,which will provide an important reference for the quality monitoring of GBE preparations.

关 键 词：银杏叶提取物 注射液 HPLC指纹图谱 液质联用 黄酮 

分 类 号：R284.1[医药卫生—中药学]