红松梢斑螟啮小蜂热激蛋白基因的筛选与鉴定  

Screening and Identification of Heat Shock Protein Genes in Baryscapus dioryctriae

作　　者：郭瑞 刘玉昆 李婧 翟淑芬 沈垭琢 张凯鹏 GUO Rui;LIU Yukun;LI Jing;ZHAI Shufen;SHEN Yazhuo;ZHANG Kaipeng(Center for Biological Disaster Prevention and Control of National Forestry and Grassland Administration,Shenyang 110034,China;First Middle School in Zhenlai County of Jilin Province,Baicheng 137300,China;Jilin Provincial Academy of Forestry Sciences,Changchun 130033,China;Jilin Provincial Key Laboratory of Animal Resources and Biodiversity in Changbai Mountain,Changchun 130033,China;Jilin Provincial Science and Technology Innovation Center for Major Forest Disease and Pest Monitoring and Prevention,Changchun 130033,China;Xinlicheng Reservoir Adiministration of Changchun City,Changchun 130119,China)

机构地区：[1]国家林业和草原局生物灾害防控中心,辽宁沈阳110034 [2]吉林省镇赉县第一中学校,吉林白城137300 [3]吉林省林业科学研究院,吉林长春130033 [4]长白山动物资源与生物多样性吉林省重点实验室,吉林长春130033 [5]吉林省主要森林病虫害监测、防疫科技创新中心,吉林长春130033 [6]长春市新立城水库管理局,吉林长春130119

出　　处：《吉林林业科技》2025年第2期17-22,共6页Journal of Jilin Forestry Science and Technology

摘　　要：热激蛋白(Hsp)在昆虫抵抗外界不良环境(如寒冷、缺氧、种群密度过大等)胁迫中发挥着重要作用。红松梢斑螟啮小蜂的生境冬季寒冷,推测其具有很强的抗寒性。为研究红松梢斑螟啮小蜂的HSPs基因家族,本文基于其全基因组数据,对其Hsps基因进行了筛选与鉴定。结果表明:共鉴定出23个Hsps基因,其中包括11个小热激蛋白(sHsps)、1个Hsp40、1个Hsp60、8个Hsp70s和2个Hsp90s。通过构建Hsps和sHsps系统发育树探讨其与其他昆虫的同源性,并利用红松梢斑螟啮小蜂Hsps在不同组织间的表达量构建热图以讨论其潜在功能。本研究可为后续进一步对红松梢斑螟啮小蜂Hsps基因功能展开研究提供理论基础和数据支撑。Heat shock protein(Hsp)played an important role in insect resistance to adverse external environments,such as cold,hypoxia,and excessive population density.The habitat of Baryscapus dioryctriae was cold in winter,so it was speculated that it had strong cold resistance.In order to study its HSPs gene family,the Hsps genes were screened and identified based on its whole genome data.The results showed that a total of 23 Hsps genes were identified,including 11 small heat shock proteins(sHsps),1 Hsp40,1 Hsp60,8 Hsp70s and 2 Hsp90s.The homology of Baryscapus dioryctriae with other insects was explored by constructing phylogenetic trees of Hsps and sHsps.The heat map was constructed using the expression of Hsps in different tissues to discuss their potential functions.This study could provide theoretical basis and data support for further research on the function of Baryscapus dioryctriae Hsps genes.

关 键 词：红松梢斑螟啮小蜂 热激蛋白 基因鉴定 

分 类 号：S763.43[农业科学—森林保护学]