加味金匮肾气丸通过Notch信号通路对肾阳虚哮喘大鼠Th1/Th2免疫失衡的影响  

Effect of Modified Jingui Shenqi Pills on Th1/Th2 Immune Imbalance in Asthma Rats with Kidney Yang Deficiency Through Notch Signaling Pathway

作　　者：蒋朱秀 李敏静 郑小伟[3] 宋红[3] 郭祥 陈家昊 JIANG Zhuxiu;LI Minjing;ZHENG Xiaowei;SONG Hong;GUO Xiang;CHEN Jiahao(Department of Respiratory Medicine,Xixi Hospital of Hangzhou,Hangzhou Zhejiang 310023,China;Department of Respiratory Medicine,the Second Affiliated Hospital of Zhejiang Chinese Medical University,Hangzhou Zhejiang 310005,China;School of Basic Medical Sciences,Zhejiang Chinese Medical University,Hangzhou Zhejiang 310053,China)

机构地区：[1]杭州市西溪医院呼吸内科,浙江杭州310023 [2]浙江中医药大学附属第二医院呼吸内科,浙江杭州310005 [3]浙江中医药大学基础医学院,浙江杭州310053

出　　处：《新中医》2025年第6期170-175,共6页New Chinese Medicine

基　　金：浙江省中医药管理局课题(2021ZB132)。

摘　　要：目的:观察加味金匮肾气丸通过Notch信号通路调控Th1/Th2失衡对肾阳虚哮喘大鼠的治疗作用。方法:按随机数字表法将40只清洁级雄性Wistar大鼠分为正常组(对照组)、肾阳虚证哮喘模型组(模型组)、加味金匮肾气丸组(中药组)及地塞米松组(西药组),每组10只。除对照组外,其余的模型组、中药组、西药组均采用氢化可地松+卵清蛋白+氢氧化铝三联法构建肾阳虚证哮喘大鼠模型;于实验第15天(即激发第1天)开始给药,西药组给予地塞米松片[0.25 mg(/kg•d)]灌胃,中药组给相应加味金匮肾气丸水煎剂[2 mg(/kg•d)]灌胃;对照组、模型组给0.9%氯化钠溶液2 mL灌胃。灌胃治疗14天,以常规苏木精-伊红(HE)染色大鼠肺组织,观察肺组织病理学改变及气道重塑变化,以酶联免疫吸附法(ELISA)测定Th1/Th2相关细胞因子,以逆转录聚合酶链式反应(RT-PCR)检测肺组织Notch1 mRNA表达。结果:与模型组比较,中药组、西药组大鼠肺组织病理损伤较模型组明显改善,炎性细胞浸润减少(P<0.05);中药组、西药组肺泡灌洗液白细胞介素-4(IL-4)、白细胞介素-5(IL-5)、白细胞介素-13(IL-13)水平均下降(P<0.05),白细胞介素-12(IL-12)和干扰素-γ(INF-γ)水平均上升(P<0.05);肺组织中Notch1 mRNA表达下降(P<0.05)。结论:加味金匮肾气丸对肾阳虚哮喘大鼠有一定的治疗作用,其机制与下调Notch1信号通路,调节肾阳虚哮喘大鼠Th1/Th2失衡相关。Objective:To observe the treatment effect of modified Jingui Shenqi Pills on Th1/Th2 immune imbalance in asthma rats with kidney yang deficiency through Notch signaling pathway.Methods:A total of 40 clean grade male Wistar rats were randomly divided into the normal group(the control group),the kidney yang deficiency asthma model group(the model group),the modified Jingui Shenqi Pills group(the Chinese medicine group),and the Dexamethasone group(the western medicine group),with 10 rats in each group.Except for the control group,the model group,the Chinese medicine group,and the western medicine group all used the triple method of Hydrogenated Dexamethasone+Ovalbumin+Aluminum Hydroxide to construct kidney yang deficiency asthma rat models;starting from the 15th day of the experiment(i.e.the first day of stimulation),the western medicine group was given Dexamethasone Tablets at a dose of 0.25 mg(/kg·d)by gavage,the Chinese medicine group was given the corresponding decoction of modified Jingui Shenqi Pills at a dose of 2 mg(/kg·d)by gavage,and the control group and the model group were given 2 mL of 0.9% sodium chloride solution by gavage.After 14 days of gastric lavage treatment,rat lung tissue was stained with conventional hematoxylin eosin(HE)to observe pathological changes and airway remodeling.Th1/Th2 related cytokines were measured by enzyme-linked immunosorbent assay(ELISA),and Notch1 mRNA expression in lung tissue was detected by reverse transcription polymerase chain reaction(RT-PCR).Results:Compared with the model group,the pathological damage of lung tissue in the Chinese medicine group and the western medicine group was significantly improved,and the infiltration of inflammatory cells was reduced(P<0.05);the levels of interleukin-4(IL-4),interleukin-5(IL-5),and interleukin-13(IL-13)in the bronchoalveolar lavage fluid in the Chinese medicine group and the western medicine group were decreased(P<0.05),while the levels of interleukin-12(IL-12)and interferon-γ(INF-γ)were increased(P<0.05);the expression

关 键 词：哮喘 肾阳虚 加味金匮肾气丸 NOTCH信号通路 Th1/Th2免疫失衡 WISTAR大鼠 

分 类 号：R562.25[医药卫生—呼吸系统]