副猪嗜血杆菌PLGA-Omp16微球对小鼠树突状细胞免疫基因表达的影响  

Effects of PLGA-Omp 16 Microspheres of Haemophilus parasuis on Immune Gene Expression of Dendritic Cells in Mice

作　　者：陈海玉 戴婷婷 廖悦辰 刘怡婷 雷天宇 庄丽云 李晓华[1,2] 郑新添 CHEN Haiyu;DAI Tingting;LIAO Yuechen;LIU Yiting;LEI Tianyu;ZHUANG Liyun;LI Xiaohua;ZHENG Xintian(Longyan University,Fujian 364000,China;Fujian Key Laboratory of Livestock Infectious Disease Prevention and Biotechnology,Longyan,Fujian 364000,China)

机构地区：[1]龙岩学院,福建龙岩364000 [2]福建省家畜传染病防治与生物技术重点实验室,福建龙岩364000

出　　处：《龙岩学院学报》2025年第2期65-70,共6页Journal of Longyan University

基　　金：福建省科技计划引导性项目(2023N0016);龙岩学院博士科研启动基金(LB2021007);2022年度武平县奇迈科技创新基金(WPQM2022013)。

摘　　要：将小鼠骨髓源树突状细胞(DCs)分别与副猪嗜血杆菌的Omp16蛋白、载Omp16的PLGA微球(PLGA-Omp16微球)以及LPS进行24 h共培养后,收集细胞提取RNA,用qPCR方法检测DCs细胞因子(IFN-γ、IL-1β、IL-6、TNF-α、INF-γ、CCL2和CXCL10)和表面标志物(MHC-II、CD80和CD86)mRNA的转录水平。结果显示,与各组抗原共培养24 h后,DCs细胞因子和表面标志物的mRNA转录水平显著高于PBS组(P<0.01);PLGA-Omp16微球组的TNF-α、IL-1β、CCL2、CXCL10、MHC-II、CD80和CD86等免疫分子mRNA转录水平显著高于Omp16组(P<0.05)。结果表明,PLGA-Omp16微球可增进DCs细胞因子转录水平,促进DCs的成熟。After co-culturing mouse bone marrow-derived dendritic cells(DCs)with the Omp 16 protein of Haemophilus parasuis,PLGA microspheres carrying Omp 16(PLGA-Omp 16 microspheres),and LPS for 24 hours,the cells were collected,and RNA was extracted.The mRNA transcription levels of DCs cytokines(IFN-γ,IL-1β,IL-6,TNF-α,INF-γ,CCL2,and CXCL10)and surface markers(MHC-II,CD80,and CD86)were detected by qPCR.The results reveal that after 24-hour coculture with each antigen group,the mRNA transcription levels of DCs cytokines and surface markers were significantly higher than those in the PBS group(P<0.01).The mRNA transcription levels of immune molecules such as TNF-α,IL-1β,CCL2,CXCL10,MHC-II,CD80,and CD86 in the PLGA-Omp 16 microsphere group were significantly higher than those in the Omp 16 group(P<0.05).The results show that PLGA-Omp16 microspheres could enhance the transcription level of DCs cytokines and promote the maturation of DCs.

关 键 词：副猪嗜血杆菌 树突状细胞 细胞因子 PLGA 表面分子 

分 类 号：S852.4[农业科学—基础兽医学]