中华常春藤茎芽苗诱导植株再生方法的建立  

Axillary Buds Induction and Plantlet Regeneration from Stem Segment Explants in Hedera nepalensis variety sinensis

作　　者：李鹏飞 潘冰容 吴丽玲 邢建宏[1] LI Pengfei;PAN Bingrong;WU Liling;XING Jianhong(Sanming University,Sanming,Fujian 365004,China;Shantou University,Shantou,Guangdong 515063,China)

机构地区：[1]三明学院/药用植物开发利用福建省高校工程研究中心,福建三明365004 [2]汕头大学,广东汕头515063

出　　处：《龙岩学院学报》2025年第2期76-84,共9页Journal of Longyan University

基　　金：福建省自然科学基金项目(2022J011173);福建省大学生创新创业训练计划项目(S202211311063)。

摘　　要：为了建立中华常春藤植株高效再生的快繁方法,选取茎尖、茎段为外植体,设置0.1%的升汞灭菌时间,以MS培养基为基本培养基,利用正交实验对茎芽苗诱导与植株再生中大量元素浓度和植物激素用量进行优化。结果表明:选取中华常春藤的茎段为外植体,出芽率高达100%。经过洗洁精预处理后,使用0.1%的升汞消毒6 min,灭菌效果最佳,污染率为1.7%,成活率为98.3%;腋芽诱导的最佳培养基为MS+6-BA 4.0 mg/L+GA_(3)1.5 mg/L+NAA 0.2 mg/L,一次诱导率可达93.3%;继代增殖的最佳培养基为MS+6-BA 2.0 mg/L+KT 0.2 mg/L+NAA 0.2 mg/L,45 d后,增值系数可达7.17;试管苗生根的最佳培养基为1/2MS+NAA 0.2 mg/L+KT 0.2 mg/L,45 d后,生根率可达85.1%;以2∶1混合的腐殖土和黄沙土为移栽基质,并在移栽前使用1.5×10^(-6)的IBA浸泡试管苗根部10 min,可显著提高成活率,移栽成活率达90.0%。To establish an efficient regeneration system for Hedera nepalensis variety sinensis.The stem tip and segment of Hedera nepalensis variety sinensis were used as explants,0.1%mercuric ascent sterilization time was set,MS medium was used as the basic medium.The concentration of the macro element and plant hormones in axillary buds induction and plantlet regeneration were optimized by orthogonal design.The results showed that the stem segments of Hedera nepalensis variety sinensis were pretreated with detergent and sterilized with 0.1%mercury for 6 min.The contamination rate was only 1.7%and the survival rate was highest at 98.3%.The optimum medium for axillary bud induction was basal MS medium supplemented with 4.0 mg/L 6-BA,1.5mg/L GA_(3) and 0.2 mg/L NAA,the initial induction rate was up to 93.3%.The optimum medium for multiplication culture was basal MS medium supplemented with 2.0 mg/L 6-BA and 0.2 mg/L KT,the multiplication time was 7.17 after 45 days.The best medium for rooting of shoots was basal 1/2 MS medium supplemented with 0.2 mg/L NAA and 0.2 mg/L KT,in which the rooting rate was 85.1%after 45 d.The plantlet was transplanted in the substrate of humus soil and yellow sand soil mixed with 2:1,and the roots of the plantlet were soaked with 1.5×10^(-6) IBA 10 min before transplanting can increase the survival rates,the survival rate of transplanting was 90.0%.

关 键 词：中华常春藤 组织培养 腋芽诱导 植株再生 

分 类 号：Q945.78[生物学—植物学]