HIV感染者/AIDS患者血浆病毒载量和外周血CD4^(+)、CD8^(+)T淋巴细胞的变化分析  

Analysis of Changes of Plasma Viral Load and Peripheral Blood CD4^(+)and CD8^(+)T Lymphocytes in HIV Infected People/AIDS Patients

作　　者：姚芳斌 李苹 邓琼 邓世华[1] YAO Fangbin;LI Ping;DENG Qiong;DENG Shihua(Department of Clinical Laboratory,First People's Hospital of Fuzhou,Fuzhou 344000,China;不详)

机构地区：[1]抚州市第一人民医院检验科,江西抚州344000

出　　处：《中国医学创新》2025年第9期124-128,共5页Medical Innovation of China

摘　　要：目的:探究人类免疫缺陷病毒(HIV)感染者/获得性免疫缺陷综合征(AIDS)患者血浆病毒载量和外周血CD4^(+)、CD8^(+)T淋巴细胞的变化。方法:根据疾病的不同将抚州市第一人民医院2022年3月-2024年3月接收的100例HIV感染者/AIDS患者分为观察1组(HIV,n=60例)、观察2组(AIDS,n=40),并选择同时期体检的健康人群50例作为此次研究对照组。采集所有研究对象的外周静脉血液,采用流式细胞仪分别检测三组CD4^(+)、CD8^(+)T淋巴细胞水平,并采用荧光定量PCR方法测定观察1组、观察2组患者血浆病毒载量。采用Pearson分析HIV感染者/AIDS患者血浆病毒载量与外周血CD4^(+)、CD8^(+)T淋巴细胞的相关性。结果:观察1组、观察2组外周血CD4^(+)水平均低于对照组,差异均有统计学意义(P<0.05),观察2组外周血CD4^(+)水平低于观察1组,差异有统计学意义(P<0.05);观察1组、观察2组外周血CD8^(+)水平均高于对照组,差异均有统计学意义(P<0.05),观察1组外周血CD8^(+)水平高于观察2组,差异有统计学意义(P<0.05);观察2组患者血浆病毒载量水平明显高于观察1组,差异有统计学意义(P<0.05);经Pearson相关性分析发现,外周血CD4^(+)水平与血浆病毒载量水平呈现明显的负相关(r=-0.685,P<0.05),而外周血CD8^(+)水平与血浆病毒载量水平无明显的相关性(r=-0.712,P>0.05)。结论:相较于健康人群,HIV感染者/AIDS患者外周血CD4^(+)T淋巴细胞偏低,而CD8^(+)T淋巴细胞偏高,且HIV感染者与AIDS患者外周血CD4^(+)、CD8^(+)T淋巴细胞水平差异明显,而随着血浆病毒载量水平的增加,HIV感染者/AIDS患者外周血CD4^(+)T淋巴细胞水平下降。Objective:To investigate the changes of plasma viral load and peripheral blood CD4^(+)and CD8^(+)T lymphocytes in human immunodeficiency virus(HIV)infected people/acquired immunodeficiency syndrome(AIDS)patients.Method:According to different diseases,100 HIV infected people/AIDS patients received from First People's Hospital of Fuzhou from March 2022 to March 2024 were divided into observation group 1(HIV infected people,n=60)and observation group 2(AIDS patients,n=40),and 50 healthy people who underwent physical examination during the same period were selected as the control group of this study.Peripheral venous blood of all object of study were collected,and the levels of CD4^(+)and CD8^(+)T lymphocytes in the three groups were detected by flow cytometry,and the plasma viral load of patients in observation group 1 and observation group 2 were measured by fluorescence quantitative PCR.Pearson was used to analyze the correlation between plasma viral load and peripheral blood CD4^(+)and CD8^(+)T lymphocytes in HIV infected people/AIDS patients.Result:The level of peripheral blood CD4^(+)in observation group 1 and observation group 2 was lower than that in control group,the differences were statistically significant(P<0.05);the level of peripheral blood CD4^(+)in observation group 2 was lower than that in observation group 1,the difference was statistically significant(P<0.05).The level of peripheral blood CD8^(+)in observation group 1 and observation group 2 was higher than that in control group,the differences was statistically significant(P<0.05);the level of peripheral blood CD8^(+)in observation group 1 was higher than that in observation group 2,the difference was statistically significant(P<0.05).The level of plasma viral load in observation group 2 was significantly higher than that in observation group 1,with statistical significance(P<0.05).Pearson correlation analysis showed that CD4^(+)level in peripheral blood was negatively correlated with plasma viral load(r=-0.685,P<0.05),while CD8^(+)level in perip

关 键 词：人类免疫缺陷病毒 获得性免疫缺陷综合征 血浆病毒载量 CD4^(+) CD8^(+) 相关性 

分 类 号：R51[医药卫生—内科学]