基于数据挖掘和网络药理学研究徐昌伟治疗腰椎间盘突出症的用药规律及作用机制  

Medication Rules and Mechanism of XU Changwei in the Treatment of Lumbar Disc Herniation Based on Data Mining and Network Pharmacology

作　　者：邓佳 赵文生 李孝林 陈洪卫[3] 彭昌华[3] 王华东 张朝驹[3] 何川 DENG Jia;ZHAO Wensheng;LI Xiaolin;CHEN Hongwei;PENG Changhua;WANG Huadong;ZHANG Chaoju;HE Chuan(College of Acupuncture and Moxibustion and Orthopedics,Hubei University of Chinese Medicine,Wuhan 430061,Hubei,China;Yangtze University Health Science Center,Jingzhou 434023,Hubei,China;Department of Orthopaedics,Jingzhou Hospital of Traditional Chinese Medicine Affiliatedto Hubei University of Chinese Medicine,Jingzhou 434000,Hubei,China)

机构地区：[1]湖北中医药大学针灸骨伤学院,湖北武汉430061 [2]长江大学医学部,湖北荆州434023 [3]湖北中医药大学附属荆州市中医医院骨科,湖北荆州434000

出　　处：《医学信息》2025年第5期16-25,共10页Journal of Medical Information

基　　金：全国名老中医专家传承工作室建设项目(编号:国中医药人教函〔2022〕75号);荆州市中医药研究所自主选题项目(编号:ZZXT2023Y08)。

摘　　要：目的探讨徐昌伟教授治疗腰椎间盘突出症的用药规律及作用机制。方法搜集徐昌伟教授2010-2018年于荆州市中医医院门诊治疗腰椎间盘突出症的病例资料,运用“中医传承辅助平台(V3.5)”对数据进行频数分析和关联分析,获得核心处方,并对其进行网络药理学分析。运用TCMSP数据库获取核心组方中药活性成分,利用Gene Cards、OMIM数据库筛选疾病靶点并生成韦恩图,使用Cytoscape3.9.2软件构建核心网络。运用Metascape在线数据库对获得的关键靶点进行GO、KEGG功能富集分析。运用Autodock程序进行对接,通过PyMOL实现结果的可视化。结果共录入200个病例,处方涉及179味中药,高频药物10味,温、平性药物运用最多,多归肝、脾、肾三经,性味以辛、苦、甘多见;关联规则得到60条数据,聚类分析得到3组核心组合。4种核心药物(川芎、当归、杜仲、牛膝)中核心活性成分共59个,靶点基因144个,疾病靶点613个,药物-疾病共同靶点共40个。GO功能富集条目3346条,其中生物过程(BP)2929条,细胞组成(CC)158条,分子功能(MF)259条;KEGG富集通路共198条。核心组方中的β-谷甾醇、6′-甲氧基-(9R)-9-醛金鸡聚糖-9-醛、黄芩苷、汉黄芩素、肉豆蔻酮等主要活性成分通过AGE-RAGE、IL-17、TNF等信号通路作用于TNF、IL6、AKT1等靶点蛋白。分子对接结果显示主要活性成分与核心靶点有着强烈结合活性。结论徐昌伟教授治疗腰椎间盘突出症重在补益肝肾、活血化瘀、祛风除湿,兼顾护脾胃,用药以温平为主,通过多个通路作用于TNF、IL6、AKT1等靶点蛋白,并通过其抗炎、抗氧化、抗凋亡、免疫调节等多种机制治疗腰椎间盘突出症。Objective To explore the medication rule and mechanism of Professor XU Changwei in the treatment of lumbar disc herniation.Methods The case data of Professor XU Changwei's treatment of lumbar disc herniation in the outpatient department of Jingzhou Hospital of Traditional Chinese Medicine from 2010 to 2018 were collected.The frequency analysis and correlation analysis of the data were carried out by using the“Traditional Chinese Medicine Inheritance Support Platform(V3.5)”to obtain the core prescription,and the network pharmacology analysis was carried out.The TCMSP database was used to obtain the active components of the core prescription,the Gene Cards and OMIM databases were used to screen the disease targets and generate the Venn diagram,and the Cytoscape 3.9.2 software was used to construct the core network.Metascape online database was used to perform GO and KEGG functional enrichment analysis on the obtained key targets.The Autodock program was used for docking,and the visualization of the results was realized through PyMOL.Results A total of 200 cases were recorded.The prescriptions involved 179 traditional Chinese medicines,10 of which were high-frequency drugs.Warm and mild drugs were the most used,mostly belonging to the three meridians of liver,spleen and kidney.Pungent,bitter and sweet were the most common tastes.60 data were obtained by association rules,and 3 core combinations were obtained by cluster analysis.There were 59 core active components,144 target genes,613 disease targets and 40 drug-disease common targets in the 4 core drugs(Chuanxiong,Danggui,Duzhong and Niuxi).There were 3346 GO functional enrichment items,including 2929 biological processes(BP),158 cell composition(CC),and 259 molecular functions(MF).There were 198 KEGG enrichment pathways.The main active ingredients such as β-sitosterol,6′-methoxy-(9R)-9-formyl-chickennan-9-aldehyde,baicalin,wogonin,and myristone in the core prescription act on TNF,IL6,AKT1 and other target proteins through AGE-RAGE,IL-17,TNF and other signal

关 键 词：名老中医经验 腰椎间盘突出症 数据挖掘 用药规律 作用机制 

分 类 号：R723.14[医药卫生—儿科]