鹅掌楸苷对小鼠慢性放射性直肠炎保护作用及其机制的研究  

Study on the protective effect of liriodendrin on chronic radiation proctitis in mice and its mechanism

作　　者：黄灏 张焰凌 贾贵清[2] 赵高平 HUANG Hao;ZHANG Yan-ling;JIA Gui-qing;ZHAO Gao-ping(The Affiliated Hospital of Southwest Medical University,Luzhou 646000,China;Department of Gastrointestinal Surgery,Sichuan Academy of Medical Sciences&Sichuan Provincial People′s Hospital(Affiliated Hospital of Univer-sity of Electronic Science and Technology of China),Chengdu 610072,China)

机构地区：[1]西南医科大学附属医院,四川泸州646000 [2]四川省医学科学院·四川省人民医院(电子科技大学附属医院)胃肠外科,四川成都610072

出　　处：《实用医院临床杂志》2025年第2期52-56,共5页Practical Journal of Clinical Medicine

基　　金：四川省科技厅重点研发项目子课题(编号:2022YFS0157)。

摘　　要：目的探究鹅掌楸苷对慢性放射性直肠炎的保护作用及其可能机制。方法将32只雌性C57/BL小鼠按照随机原则分为空白对照组、模型组、鹅掌楸苷灌胃组以及鹅掌楸苷灌肠组四组,每组8只小鼠。采用10 Gy X射线单次局部辐照小鼠盆腔区域建立慢性放射性直肠炎模型,鹅掌楸苷灌胃组和鹅掌楸苷灌肠组分别予以100 mg/kg鹅掌楸苷灌胃或灌肠,模型组与空白对照组予以等体积生理盐水灌胃,每周1次,持续8周。观察各组小鼠体重与排便情况,评估小鼠生存情况并评分,绘制生存曲线;HE染色观察小鼠肠组织病理学变化并统计病理评分;ELISA检测小鼠血清中促炎因子肿瘤坏死因子-α(TNF-α)、干扰素-γ(IFN-γ),抑炎因子白细胞介素-10(IL-10)、转化生长因子-β1(TGF-β1)水平。结果鹅掌楸苷灌胃组和灌肠组小鼠生存率均高于模型组(P<0.05),其中,鹅掌楸苷灌胃组与灌肠组小鼠体重与排便情况均优于模型组(P<0.05);与模型组相比,鹅掌楸苷灌胃组和灌肠组小鼠血清中促炎因子IFN-γ、TNF-α水平明显降低,抑炎因子TGF-β1、IL-10水平明显升高(P<0.05)。结论鹅掌楸苷能有效改善小鼠慢性放射性直肠炎的炎症反应、保护直肠组织,提高慢性放射性直肠炎小鼠生存率,改善小鼠辐照后生存情况,其作用机制可能与鹅掌楸苷调控炎症相关细胞因子的释放有关。Objective To explore the protect effect of liriodendrin on chronic radiation proctitis and its possible mechanism.Methods Thirty-two female C57/BL mice were randomly divided into a blank control group,a model group,a liriodendrin gavage group and a liriodendrin enema group,8 in each group.Expect the blank control group,the other groups were subjected to a single local irradiation of 10 Gy X-rays in the pelvic area of mice to establish a mouse model of chronic radiation enteritis.The liriodendrin gavage group was given 100 mg/kg liriodendrin.The liriodendrin enema group was given 100 mg/kg liriodendrin.The model group and the blank control group were given the same amount of normal saline via gavage once a week for 8 weeks.The weight and defecation of mice in each group were observed.The survive status of mice was evaluated and scored.The survival curve was drawn.The pathological changes in intestinal tissue were observed by using HE staining,and the pathological score was calculated.The levels of pro-inflammatory factors such as TNF-α、IFN-γand anti-inflammatory cytokines such as IL-10 and TGF-β1 in the serum of mice were detected by using ELISA method.Results The survival rate of mice in the liriodendrin gavage group and enema group was higher than that in the model group.Additionally,the body weight and defecation of mice in the liriodendrin gavage group and enema group were better than those in the model group(P<0.05).Compared with the model group,the levels of pro-inflammatory factors IFN-γand TNF-αin the intestinal tissue of mice in the liriodendrin gavage group and enema group were significantly decreased,while the levels of anti-inflammatory factors TGF-βand IL-10 were significantly increased(P<0.05).Conclusions Liriodendrin can effectively inhibit the inflammatory response of chronic radiation proctitis in mice and protect rectal tissue.It can improve the survival rate of mice with chronic radiation proctitis and improve the survival of mice after irradiation.The mechanism may be related to the reg

关 键 词：慢性放射性直肠炎 鹅掌楸苷 细胞因子 

分 类 号：R817.1[医药卫生—影像医学与核医学]