KRAS G12、LHCGRinsLQ突变与卵巢子宫内膜异位症不孕的研究  

Study on mutations of KRAS G12 and LHCGR insLQ and ovarian endometriosis infertility

作　　者：周鑫 兰婷婷 郭春 刘小琦[3] ZHOU Xin;LAN Ting-ting;GUO Chun;LIU Xiao-qi(School of Medicine,University of Electronic Science and Technology of China,Chengdu 610054,China;Reproductive Center,Sichuan Academy of Medical Sciences&Sichuan Provincial People's Hospital(Affiliated Hospital of University of Electronic Science and Technology of China),Chengdu 610072,China;Medical Genetics Center,Sichuan Academy of Medical Sciences&Sichuan Provincial People's Hospital(Affiliated Hospital of University of Electronic Science and Technology of China),Chengdu 610072,China)

机构地区：[1]电子科技大学医学院,四川成都610054 [2]四川省医学科学院·四川省人民医院(电子科技大学附属医院)妇产科生殖中心,四川成都610072 [3]四川省医学科学院·四川省人民医院(电子科技大学附属医院)医学遗传中心,四川成都610072

出　　处：《实用医院临床杂志》2025年第2期61-65,共5页Practical Journal of Clinical Medicine

基　　金：四川省省级科技计划项目(编号:2022YFS0085)。

摘　　要：目的研究卵巢内异症不孕患者和输卵管性不孕患者KRAS G12、LHCGR insLQ突变及颗粒细胞中KRAS表达水平差异,探索内异症不孕机制。方法以40例卵巢内异症不孕患者为病例组,42例输卵管性不孕症患者为对照组,比较两组KRAS G12、LHCGR insLQ突变及颗粒细胞KRAS表达水平。结果所有样本均未发现KRAS G12突变,颗粒细胞KRAS表达差异无统计学意义(P=0.438)。病例组LHCGR insLQ突变率为7.50%(3/40),对照组为2.38%(1/42),差异无统计学意义(P=0.353)。结论本研究表明颗粒细胞似乎不会通过KRAS突变影响女性生殖过程,从而造成卵巢子宫内膜异位症患者不孕。两组LHCGR insLQ突变率虽有一定差异,病例组呈现突变率较高的趋势,但未观察到统计学差异,需扩大样本量进一步研究。Objective To investigate the mutations of KRAS G12 and LHCGR insLQ and the difference of KRAS expression levels in granulosa cells in patients with intraovarian infertility and tubal infertility,and explore the infertility mechanism of intraovarian infertility.Methods Forty infertile patients with ovarian endometriosis were selected as a case group and 42 patients with fallopian tube infertility were selected as a control group.The KRAS G12 and LHCGR insLQ mutations and KRAS expression levels in granulosa cells were compared between the two groups.Results No KRAS G12 mutation was found in all samples,and there was no significant difference in KRAS expression in granulosa cells(P=0.438).The mutation rate of LHCGR insLQ was 7.50%(3/40)in the case group and 2.38%(1/42)in the control group.The difference was no statistically significant(P=0.353).Conclusions This study shows that granulosa cells do not appear to affect the female reproductive process through KRAS mutations,resulting in infertility in ovarian endometriosis patients.Although there is a certain difference in LHCGR insLQ mutation rate between the two groups,the mutation rate shows a higher trend in the case group,but no statistical difference was observed.Further study should be conducted with larger sample size.

关 键 词：子宫内膜异位症 不孕 KRAS基因 LHCGR基因 基因突变 

分 类 号：R711.6[医药卫生—妇产科学]