配位策略制备的类漆酶纳米酶及其分析应用  

Laccase-like Nanozyme Prepared with Coordination Strategy and Their Analytical Applications

作　　者：王斌富 张紫若 高启 徐豪迪 李文英 童丁毅 WANG Bin-Fu;ZHANG Zi-Ruo;GAO Qi;XU Hao-Di;LI Wen-Ying;TONG Ding-Yi(School of Materials Science and Chemical Engineering,Ningbo University,Ningbo 315201,China;School of Mechanical Engineering,Hangzhou Dianzi University,Hangzhou 310018,China)

机构地区：[1]宁波大学材料科学与化学工程学院,宁波315201 [2]杭州电子科技大学机械学院,杭州310018

出　　处：《分析化学》2025年第2期164-175,共12页Chinese Journal of Analytical Chemistry

基　　金：浙江省教育厅项目(No.Y202351659);宁波市自然科学基金项目(No.202003N4078);杭州电子科技大学科研启动基金项目(No.KYS015624299)资助。

摘　　要：漆酶是一种多酚氧化酶,可以催化酚类、芳香胺类和儿茶酚胺类等化合物的氧化,在污染物降解和分析检测领域有广泛的应用。但是,天然漆酶制备成本高,其活性受环境因素的影响较大,易变性失活,影响了其实际应用效果。纳米酶是一类具有类天然酶活性的纳米材料,具有易于制备、活性可调和稳定性高等优点。目前,研究者已经开发出多种纳米酶。天然漆酶活性位点的Cu^(2+)与氨基酸配位,受此启发,研究者将配位合成策略即基于金属离子和配体相互作用的策略用于类漆酶纳米酶的制备。在已报道的采用配位策略制备的类漆酶纳米酶中,金属节点通常为铜、锰和铈等,配体涉及多类化合物(包括核苷酸、氨基酸、多肽和芳香酸等),可通过调控金属与配体的比例、配体的还原能力、缓冲溶液、氯离子和溴离子等因素实现对类漆酶纳米酶催化活性的调节。本文分类综述了采用配位策略合成的类漆酶纳米酶及其在检测酚类化合物、疾病标志物、抗生素、农药、含硫污染物以及时间-温度指示等领域的应用,讨论了当前类漆酶纳米酶研究中存在的问题,并展望了其未来的发展趋势。Laccase is a type of polyphenol oxidase that can catalyze the oxidation of various substances,including phenols,aromatic amines,and catecholamines.It has been widely utilized in pollutant degradation and analytical applications.However,the high cost of preparation of natural laccase and its susceptibility to environmental factors,which can lead to denaturation and inactivation,limit its practical applications.Nanozymes,which are nanomaterials that exhibit enzyme-like properties,offer advantages such as easy preparation,adjustable activity,and exceptional stability.Currently,many types of nanozymes have been developed.Inspired by the coordination of Cu^(2+)with amino acids in the active site of natural laccase,researchers have employed coordination synthetic strategies to prepare laccase-like nanozymes.The metal nodes in these laccase-like nanozymes include copper,manganese,and cerium,while the ligands involve a variety of chemicals like nucleotides,amino acids,polypeptides,and aromatic acids.By manipulating factors such as the metal-to-ligand ratio,reducing capacity of ligands,buffer solutions,chloride ions,bromine ions,the catalytic activity of laccase-like nanozymes can be finely tuned.In this paper,laccase-like nanozymes developed through coordination strategies were categorized and summarized,along with review of their analytical applications in detection of phenolic compounds,disease biomarkers,antibiotics,pesticides,sulfur-containing pollutants,and time-temperature indicators.Furthermore,the challenges currently faced in the research of laccase-like nanozymes and future research directions were discussed.

关 键 词：漆酶 类漆酶纳米酶 配位策略 分析应用 评述 

分 类 号：O657[理学—分析化学] TB383.1[理学—化学] O641.4[一般工业技术—材料科学与工程]