预制纳米碳-铂复合基质用于表面辅助激光解吸电离质谱成像分析植物组织中的小分子成分  

Prefabricated Nano Carbon-Platinum Composite Matrix for Surface-assisted Laser Desorption Ionization Mass Spectrometry Imaging of Small Molecular Components in Plant Tissues

作　　者：杨帆 沈钰琳 龚灿 刘兆鑫 郭强胜 许旭 YANG Fan;SHEN Yu-Lin;GONG Can;LIU Zhao-Xin;GUO Qiang-Sheng;XU Xu(School of Chemical and Environmental Engineering,Shanghai Institute of Technology,Shanghai 201418,China)

机构地区：[1]上海应用技术大学化学与环境工程学院,上海201418

出　　处：《分析化学》2025年第2期204-213,共10页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(No.31671928);上海应用技术大学科技发展基金项目(No.KJFZ2023-11)资助。

摘　　要：与使用有机小分子基质的基质辅助激光解吸电离质谱(MALDI-MS)技术相比,基于纳米材料基质的表面辅助激光解吸电离质谱(SALDI-MS)对小分子化合物的分析效果更好。本研究采用离子溅射仪将碳/铂材料溅射到玻璃盖玻片上制备纳米碳-铂(C-Pt)复合基质,建立了基于预制碳-铂复合基质的SALDI-MS分析方法。实验结果表明,使用C-Pt复合基质显著提高了待测成分质谱峰的信号强度和信噪比。考察了碳与铂的比例、离子溅射的时长和质谱激光强度的影响,得到C-Pt预制基质的最佳制备条件和质谱分析条件。在预制C-Pt复合基质上点样,采用SALDI-MS分析蜜三糖和大豆苷元,质谱峰强度点内重复性(相对标准偏差,RSD)≤4.8%,点间重复性RSD≤6.4%;在0.05~1.0 mg/mL范围内,槲皮素和蜜三糖的质谱峰强度与浓度的线性相关系数(R^(2))>0.994,显示出较好的定量分析及用于质谱成像的潜力。在预制C-Pt复合基质上点样大豆50%乙醇提取液用于SALDI-MS分析,从提取液中识别出寡糖和甘油三酯等15种化合物。在预制C-Pt复合基质上覆盖玉米组织切片,用于SALDI-MS成像分析,获得了玉米中寡糖、甘油二酯和甘油三酯的分布图,结果显示,甘油二酯和甘油三酯主要分布在玉米的胚及胚的周围,寡糖的分布较为均匀。Compared with matrix-assisted laser desorption ionization mass spectrometry(MALDI-MS)using organic small molecule matrix,surface-assisted laser desorption ionization mass spectrometry(SALDI-MS)based on nanomaterial matrix is more effective in analysis of small molecule compounds.Ion sputtering instruments have obvious advantages for applying inorganic nanomatrix.In this work,the carbon-platinum material was sputtered onto a glass cover slip using an ion sputtering instrument to form a carbon-platinum(C-Pt)composite nanomatrix,and an SALDI-MS analytical method was thus established based on the prefabricated C-Pt composite matrix.The experimental results showed that the C-Pt composite nanomatrix could significantly improve the signal intensity and signal-to-noise ratio of the mass spectrum peaks of the components to be measured.The ratio of carbon to platinum,the duration of ion sputtering,and the laser power in mass spectrometer were investigated to select the optimal C-Pt matrix prefabricated conditions and SALDI-MS experimental conditions.Using the prefabricated C-Pt composite matrix,the melittriose and daidzein sample solution were applied as sample to examine repeatability.The results showed that the intra-point repeatability(RSD)was≤4.8%and the inter-point repeatability(RSD)was≤6.4%.The quercetin and melitriose were applied as model samples,and a linearity between MS peak intensity and respective concentration in the range of 0.05-1.0 mg/mL was found,with linear correlation coefficients(R^(2))greater than 0.994,showing good potential for quantitative and imaging analysis.Then,the prefabricated C-Pt composite matrix was applied to SALDI-MS analysis of the 50%ethanol extract of soybean,and 15 kinds of compounds including oligosaccharides and triglycerides were identified from the mass spectra.Furthermore,the CPt matrix was employed in SALDI-MS imaging for the compositions in corn tissue section,and the results showed that diglycerides and triglycerides were mainly distributed in the corn embryo and around t

关 键 词：表面辅助激光解吸电离质谱 质谱成像 预制基质 纳米碳-铂复合基质 

分 类 号：Q946[生物学—植物学] O657.63[理学—分析化学]