水杨酰腙型高选择性铝离子荧光探针的合成及其应用研究  

Synthesis and Application of Salicylhydrazone Probe for Highly Selective Detection of Al^(3+)

作　　者：谭浩雪 王忠龙 杨晓琴[1] 饶小平 赵平[1] 姜倩[1] TAN Hao-Xue;WANG Zhong-Long;YANG Xiao-Qin;RAO Xiao-Ping;ZHAO Ping;JIANG Qian(Key Laboratory of State Forestry and Grassland Administration on Highly-Efficient Utilization of Forestry Biomass Resources in Southwest China,Southwest Forestry University,Kunming 650224,China;Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources,College of Chemical Engineering,Nanjing Forestry University,Nanjing 210037,China;College of Chemical Engineering,Huaqiao University,Xiamen 361021,China)

机构地区：[1]西南林业大学,西南地区林业生物质资源高效利用国家林业和草原局重点实验室,昆明650224 [2]南京林业大学化学工程学院,江苏省林业资源高效加工利用协同创新中心,南京210037 [3]华侨大学化工学院,厦门361021

出　　处：《分析化学》2025年第2期214-223,共10页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(No.32160350);云南省基础研究项目(Nos.202201AU070069,202301BD070001-117)资助。

摘　　要：本研究以香草醛衍生物为原料合成了4种水杨酰腙型化合物(L1~L4),采用核磁共振(NMR)和高分辨质谱(HRMS)对其结构进行表征。光学实验结果表明,探针L1和L3可作为铝离子(Al^(3+))荧光探针,加入Al^(3+)后,探针L1溶液的荧光颜色由无色变为蓝色,检出限为25.1 nmol/L。与探针L1相比,探针L3与Al^(3+)络合后溶液荧光颜色由无色变色绿色,检出限为17.3 nmol/L。探针L1和L3在Al^(3+)检测方面具有响应时间短、选择性好和抗干扰能力强等特点。通过HRMS和1H NMR证明了探针L1和L3对Al^(3+)的识别机理。细胞成像实验结果表明,探针L1和L3的细胞毒性低,在检测生物体内Al^(3+)方面具有较大的应用潜力。In this work,four salicylhydrazone compounds(L1-L4)were designed and synthesized by using vanillin derivatives as raw material.The structures were confirmed by nuclear magnetic resonance(NMR)and high-resolution mass spectrometry(HRMS).The optical experiments showed that probe L1 and probe L3 could be used as aluminium ion(Al^(3+))fluorescence probes.The fluorescence color of probe L1 solution changed from colorless to blue after adding Al^(3+),and the limit of detection was 25.1 nmol/L.Compared with probe L1,the fluorescence color of probe L3 solution changed from colorless to green after complexing with Al^(3+),and the limit of detection was 17.3 nmol/L.Probe L1 and probe L3 showed the advantages of fast response speed,high selectivity and good anti-interference.The mechanism of Al^(3+)recognition was further demonstrated by HRMS and 1H NMR.Cell imaging experiments showed that probe L1 and L3 had low cytotoxicity and had great application potential in detection of Al^(3+)in vivo.

关 键 词：荧光探针 水杨酰腙型化合物 铝离子 识别 细胞成像 

分 类 号：O62[理学—有机化学]