萱草花大孔树脂70%乙醇洗脱流分化学成分研究  

Study on Chemical Constituents of 70%Ethanol Elution Flow Fraction of Glycopolycarpus Macroporous Resin from Hemerocallis citrina Baroni

作　　者：陈莹 李欢 马世成 陈浩 陈雨露 李昱彤 王轶翔 史林燕 马天成 CHEN Ying;LI Huan;MA Shicheng;CHEN Hao;CHEN Yulu;LI Yutong;WANG Yixiang;SHI Linyan;MA Tiancheng(School of Pharmacy,Qiqihar Medical University,Qiqihar 161006,China)

机构地区：[1]齐齐哈尔医学院药学院,黑龙江齐齐哈尔161006

出　　处：《生物化工》2025年第1期108-112,共5页Biological Chemical Engineering

基　　金：齐齐哈尔医学院大学生创新创业训练计划项目(X202311230012)。

摘　　要：目的:对萱草花(Hemerocallis citrina Baroni)化学成分进行分离鉴定。方法:使用80%乙醇水溶液对萱草花进行冷浸提取,提取物经大孔树脂柱、硅胶柱、LH-20凝胶柱和半制备高效液相色谱法进行分离,采用紫外、质谱及核磁共振波谱对化合物进行结构鉴定。结果:从萱草花大孔树脂70%乙醇洗脱流分中分离鉴定出11个化合物,包括6个脂肪酸类、2个蒽醌类、2个二氢茋类和1个对羟基肉桂酰酯类化合物。其中,化合物(+)-(9R,10E,12E)-9-甲氧基十八碳二烯酸、1,16-二羟基十六聚糖-7-基-7-O-反式-对香豆酸酯、9,12,13-三羟基-10(E)-十八碳烯酸、9,12,13-三羟基十八碳-10(E),15(Z)-二烯酸为首次从萱草属植物中分离得到。结论:通过对萱草花的化学成分进行研究,丰富了萱草花的化学组成及其可能发挥药效的物质基础。Objective:To isolate and identify the chemical constituents of Hemerocallis citrina Baroni.Methods:The cold maceration extraction of Hemerocallis citrina Baroni is carried out with 80%ethanol-water.The extract is then separated by macroporous resin column,silica gel column,LH-20 gel column and semi-preparative high performance liquid chromatography.The structures of the compounds are identified by ultraviolet spectroscopy,mass spectrometry and nuclear magnetic resonance spectroscopy.Results:Eleven compounds are isolated and identified from the 70%ethanol elution fraction of the macroporous resin of Hemerocallis citrina Baroni,including six fatty acid compounds,two anthraquinone compounds,two dihydrostilbene compounds and one p-hydroxycinnamoyl ester compound.Among them,compounds(+)-(9R,10E,12E)-9-methoxyoctadecadienoicacid,1,16-dihydroxyhexadecan-7-yl-7-O-transp-coumarate,9,12,13-trihydroxy-10(E)-octadecenoicacid,9,12,13-trihydroxyoctadeca-10(E),15(Z)-dienoicacid are isolated from plants of the genus Hemerocallis for the first time.Conclusion:The study on the chemical constituents of Hemerocallis citrina Baroni has enriched the chemical composition of Hemerocallis citrina Baroni and its possible material basis for exerting pharmacological effects.

关 键 词：萱草花 脂肪酸 蒽醌 化学成分 

分 类 号：Q946.8[生物学—植物学]