女贞子水提物对CCl_(4)诱导小鼠亚急性肝损伤的保护作用及机制研究  

Protective Effects and Mechanisms of Ligustrum lucidum Water Extract on CCl_(4)-Induced Subacute Liver Injury in Mice

作　　者：方明[1] 蒋凯[1] 乔乐天 李向阳[1] FANG Ming;JIANG Kai;QIAO Letian;LI Xiangyang(Shangqiu Medical College,Shangqiu 476100,China)

机构地区：[1]商丘医学高等专科学校,河南商丘476100

出　　处：《生物化工》2025年第1期132-136,共5页Biological Chemical Engineering

基　　金：河南省高等学校重点科研项目计划(24B310005)。

摘　　要：目的:探究女贞子水提物对CCl_(4)诱导小鼠亚急性肝损伤的保护作用及机制。方法:将昆明种小鼠均分为5组(对照组、模型组、低剂量组、高剂量组和联苯双酯组),除对照组外其他实验组均构建肝损伤模型,给予相应处理后使用试剂盒检测多项指标,包括组织病理学、血清肝功能及肝组织纤维化、氧化应激、炎症等指标。结果:HE染色结果显示,模型组小鼠肝小叶结构破坏,肝细胞坏死,炎症细胞浸润明显,而女贞子水提物干预后,肝细胞坏死和炎症细胞浸润显著减少,高剂量组效果更佳。血清肝功能指标显示,女贞子水提物显著降低了肝损伤小鼠的谷丙转氨酶(ALT)、谷草转氨酶(AST)和总胆红素(TBIL)水平(p<0.01)。氧化应激指标显示,女贞子水提物显著降低了肝组织丙二醛(MDA)含量,提高了超氧化物歧化酶(SOD)活性(p<0.01),表明其具有显著的抗氧化作用。炎症因子白细胞介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)水平显著降低,提示女贞子水提物能够有效抑制炎症反应。此外,女贞子水提物在基因水平上抑制了肝纤维化相关因子,包括Ⅰ型胶原蛋白alpha1链(Col1a1)、Ⅲ型胶原蛋白alpha1链(Col3a1)、纤连蛋白(FN)、转化生长因子β受体2(Tgfbr2)和α-平滑肌肌动蛋白(α-SMA)的表达(p<0.01)。结论:女贞子水提物对肝损伤疗效显著,可改善肝功能、减轻炎症与纤维化,作用机制可能与调节抗氧化酶等相关,有望用于临床肝病治疗或辅助治疗。Objective:To investigate the protective effects and mechanisms of Ligustrum lucidum water extract on CCl_(4)-induced subacute liver injury in mice.Methods:Kunming mice are divided into groups(control group,model group,low-dose group,high-dose group and bifendiester group),liver injury models are established except for the control group,and after corresponding treatments,multiple indicators are detected using kits,including histopathology,serum liver function,liver tissue fibrosis,oxidative stress,inflammation and other indicators.Results:The results of HE staining show that the hepatic lobular structure is destroyed,hepatocyte necrosis and inflammatory cell infiltration are obvious in the model group,while the hepatocyte necrosis and inflammatory cell infiltration are significantly reduced after the intervention of Ligustrum lucidum water extract,and the effect is better in the high-dose group.Serum liver function indexes show that the Ligustrum lucidum water extract significantly reduced the levels of alanine aminotransferase(ALT),aspartate aminotransferase(AST)and total bilirubin(TBIL)in mice with liver injury(p<0.01).The results of oxidative stress show that the Ligustrum lucidum water extract significantly reduce the content of malondialdehyde(MDA)in liver tissue and increase the activity of superoxide dismutase(SOD)(p<0.01),indicating that it has a significant antioxidant effect.The levels of inflammatory cytokines interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)are significantly reduced,suggesting that the Ligustrum lucidum water extract could effectively inhibit the inflammatory response.In addition,the Ligustrum lucidum water extract inhibite the expression of liver fibrosisrelated factors,including type I collagen alpha1 chain(Col1a1),typeⅢcollagen alpha1 chain(Col3a1),fibronectin(FN),transforming growth factorβreceptor 2(Tgfbr2),andα-smooth muscle actin(α-SMA)at the gene level(p<0.01).Conclusion:Ligustrum lucidum water extract has a significant curative effect on liver injury,which can impro

关 键 词：CCl_(4) 胶原沉积 女贞子 肝功能 亚急性肝损伤 

分 类 号：R96[医药卫生—药理学] R932[医药卫生—药学]