外种皮对华重楼种子贮藏过程生理生化变化的影响  

Effects of Episperm on Physiological and Biochemical Changes of Paris polyphylla Seed During Storage

作　　者：王丽玲[1] 雷小华 柏明娥[1] 成亮 WANG Liling;LEI Xiaohua;BAI Ming’e;CHENG Liang(Zhejiang Academy of Forestry,Hangzhou 310023,China;Liandu Ecological Forestry Development Center,Lishui 323000,China)

机构地区：[1]浙江省林业科学研究院,浙江杭州310023 [2]莲都区生态林业发展中心,浙江丽水323000

出　　处：《浙江林业科技》2025年第1期65-72,共8页Journal of Zhejiang Forestry Science and Technology

摘　　要：探究外种皮对华重楼种子贮藏过程中生理生化指标的影响,为华重楼种子的贮藏方式提供理论依据。以带外种皮和去外种皮2种不同处理方式的华重楼种子为试验材料,分析了0、4、8、10个月贮藏期种子中抗氧化酶活性、活性氧和内源激素含量等生理生化指标的变化。结果表明:种子贮藏过程中超氧化物歧化酶(SOD)活性和苯丙氨酸酶(PAL)活性降低,去除外种皮可以减缓SOD和PAL活性的下降幅度;带种皮种子的过氧化物酶(POD)活性呈下降趋势,而去种皮种子的POD活性呈上升趋势;贮藏前,带外种皮的种子的生长类激素吲哚乙酸、赤霉素3、细胞分裂素(激动素、玉米素核苷、二氢玉米素和反式玉米素)的含量显著高于去种皮种子,而赤霉素7、1-萘乙酸、水杨酸、茉莉酸、脱落酸的含量显著低于去种皮种子。随着贮藏时间的延长,2种处理方式的种子中吲哚乙酸含量均显著上升,而其他激素含量均显著下降,说明生长类激素吲哚乙酸可能是促进华重楼种子萌发的重要内源激素之一。In order to explore the influence of epispermon physiological and biochemical indexes during seed storage of Paris polyphylla,the changes of antioxidant enzyme activities,reactive oxygen species and endogenous hormone in seeds with and without episperm were studied during 0,4,8 and 10 months of storage.The results showed that superoxide dismutase(SOD)and phenylalaninase(PAL)activities decreased during seed storage,and removing episperm could slow down the decrease of SOD and PAL activities.The POD activity of seed with episperm decreased,while that of seed without episperm increased.Before storage,the contents of auxin(indole acetic acid),gibberellin 3 and cytokinin(kinetin,zeanoside,dihydrozein and trans zein)with episperm were significantly higher than those in seeds without seedcoat,while the contents of gibberellin 7,1-naphthalene,salicylic acid,jasmonic acid and abolic acid were significantly lower than those in seeds without episperm.With the extension of storage time,the contents of indole acetic acid in the seeds of the two treatments increased significantly,while the other hormones decreased significantly,indicating that indoleacetic acid,a growth hormone,may be one of the important endogenous hormones to promote seed germination.Therefore,seed storage by removing seed coat and indoleacetic acid treatment is beneficial to seed germination.

关 键 词：华重楼 外种皮 种子贮藏 生理生化 激素 

分 类 号：S567.23[农业科学—中草药栽培]