周氏啮小蜂线粒体基因组测定与分析  

Sequencing and Analysis of Mitochondrial Genome of Chouioia cunea

作　　者：饶何燕 李响亮 卫欣怡 孙季豪 王旭[1] 黄羿鑫 RAO Heyan;LI Xiangliang;WEI Xinyi;SUN Jihao;WANG Xu;HUANG Yixin(Anhui Normal University,Anhui Wuhu 241000)

机构地区：[1]安徽师范大学,安徽芜湖241000

出　　处：《浙江林业科技》2025年第1期87-95,共9页Journal of Zhejiang Forestry Science and Technology

基　　金：国家自然科学基金项目(32100355,32100352)、安徽师范大学大学生创新创业训练计划项目。

摘　　要：本文通过Illumina二代测序技术对周氏啮小蜂Chouioia cunea线粒体基因组进行测序、组装和注释,并分析其结构特点和碱基组成;使用最大似然法重建系统发育树,分析姬小蜂科Eulophidae内的系统发育关系。结果表明,周氏啮小蜂线粒体基因组序列全长14704 bp,包含13个蛋白质编码基因、22个tRNA基因和2个rRNA基因。基因组全序列的AT含量为85.0%,表现出明显的AT偏向性。13个蛋白质编码基因均以典型的ATN为起始密码子,以TAA为终止密码子。除trnS1和trnP基因外,其余20个tRNAs的二级结构均为典型的三叶草结构,二级结构中有U-U碱基错配现象。系统发育分析显示,姬小蜂科的所有物种聚在同一分支,支持该科的单系性;周氏啮小蜂与亮腹釉小蜂Tamarixia radiata聚为一支,在系统发育树上关系最近。本研究在线粒体基因组水平探讨周氏啮小蜂的线粒体基因组结构特征,分析其在姬小蜂科内的分类地位,为揭示姬小蜂科昆虫的系统发育和进化关系提供理论依据。Chouioia cunea belongs to the family Eulophidae and is an important endoparasitic natural enemy of the pupal stage of Hyphantria cunea.It possesses a high parasitism rate and strong fecundity.In this paper,the mitochondrial genome of Chouioia cunea was sequenced,assembled,and annotated using Illumina next-generation sequencing technology,and its structural characteristics and base composition were analyzed.The maximum likelihood method was employed to reconstruct the phylogenetic tree and analyze the phylogenetic relationships within Eulophidae.The results showed that the mitochondrial genome sequence of Chouioia cunea was 14,704 bp in length,including 13 protein-coding genes,22 tRNA genes,and 2 rRNA genes.The AT content of the whole genome sequence was 85.0%,indicating a significant AT bias.All 13 protein-coding genes have the typical ATN as the start codon and TAA as the stop codon.Except for the trnS1 and trnP genes,the secondary structures of the other 20 tRNAs exhibited a typical cloverleaf structure,and a U-U base mismatch was found in the secondary structure.Phylogenetic analysis indicated that all species of Eulophidae formed a single clade,supporting the monophyly of the family.Chouioia cunea is closely related to Tamarixia radiata in the phylogenetic tree.In this study,the mitochondrial genome structure and taxonomic status of Chouioia cunea were analyzed at the mitochondrial genome level,providing a theoretical basis for understanding the phylogenetic and evolutionary relationships of Eulophidae.

关 键 词：周氏啮小蜂 线粒体基因组 全序列测定 系统发育关系 

分 类 号：Q969.545.3[生物学—昆虫学]