八珍汤对自然衰老果蝇改善作用的研究  

Study on the improving effect of Bazhen decoction on naturally aging drosophila melanogaster

作　　者：张力元 马慧颖 杨秉佳 高祥云 宾佳宜 杨利敏[1,2] 朱明雪 ZHANG Liyuan;MA Huiying;YANG Bingjia;GAO Xiangyun;BIN Jiayi;YANG Limin;ZHU Mingxue(Department of Human Anatomy and Histology,Medical College of Dalian University,Dalian 116622,China;Chronic Disease Research Center of Dalian,Dalian 116622,China;Department of Psychiatry,Dalian Medical University,Dalian 116044,China)

机构地区：[1]大连大学医学院人体解剖与组织胚胎学教研室,辽宁大连116622 [2]大连市慢性病研究中心,辽宁大连116622 [3]大连医科大学,辽宁大连116044

出　　处：《黑龙江医药科学》2025年第3期1-6,10,共7页Heilongjiang Medicine and Pharmacy

基　　金：国家自然科学基金,编号:31571087;大连市高层次人才创新支持计划青年科技之星项目,编号:2021QR034。

摘　　要：目的:探讨八珍汤(Bazhen decoction)对自然衰老果蝇的改善作用。方法:采用爬管实验,计算攀爬指数,分析八珍汤对果蝇运动能力的影响;通过记录果蝇产卵数量,分析八珍汤对果蝇生殖能力的影响;通过绘制果蝇自然死亡生存曲线,分析八珍汤对果蝇寿命的影响;利用酶联免疫吸附实验,检测果蝇体内氧化应激相关生化指标,包括超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和活性氧(ROS),以评估八珍汤的抗氧化功能。结果:0.1 g/mL浓度的八珍汤显著延长果蝇的寿命(P<0.001),平均生存期、中位生存期、最高生存期以及延寿率均有显著提升;此外,八珍汤还能够提高果蝇的运动能力(P<0.05)及繁殖能力(P<0.01),并显著提高果蝇体内SOD活力、CAT水平,降低ROS含量(P<0.05)。结论:八珍汤可通过抗氧化应激、调节氧化还原发挥抗衰老功效。Objective:To investigate the improvement effect of Bazhen decoction on naturally aging drosophila melanogaster.Methods:The climbing tube test was used to calculate the climbing index and analyze the effect of Bazhen decoction on the motor ability of drosophila melanogaster.The egg-laying number of drosophila melanogaster was recorded to analyze the effect of Bazhen decoction on their reproductive ability.The natural death survival curve of drosophila melanogaster was plotted to analyze the effect of Bazhen decoction on their lifespan.Enzyme-linked immunosorbent assay(ELISA)was used to detect oxidative stress-related biochemical indicators in drosophila melanogaster,including superoxide dismutase(SOD),catalase(CAT)and reactive oxygen species(ROS),to evaluate the antioxidant function of Bazhen decoction.Results:Bazhen Decoction at a concentration of 0.1 g/mL significantly prolonged the lifespan of drosophila melanogaster(P<0.001),with significant improvements in mean survival time,median survival time,maximum survival time and life extension rate.Additionally,Bazhen decoction improved the motor ability(P<0.05)and reproductive ability(P<0.01)of drosophila melanogaster,and significantly increased SOD activity and CAT levels while decreasing ROS content in drosophila melanogaster(P<0.05).Conclusion:Bazhen decoction exerts anti-aging effects by anti-oxidative stress and regulating redox status.

关 键 词：果蝇 衰老 八珍汤 氧化应激 

分 类 号：R392[医药卫生—免疫学] R446.5[医药卫生—基础医学]