两种石斛属植物低极性成分GC-MS分析及抗肿瘤活性研究  

GC-MS Analysis of Low-polar Compounds and Antitumor Activity Test from Two Species of Dendrobium Plants

作　　者：程忠泉 陈伟洪 周伟平 李智斌 杨丹 Cheng Zhongquan;Chen Weihong;Zhou Weiping;Li Zhibin;Yang Dan(Guangzhou Health Science College,Guangzhou 510450,China;Guangdong Medical University,Dongguan 523808,China)

机构地区：[1]广州卫生职业技术学院,广东广州510450 [2]广东医科大学,广东东莞523808

出　　处：《广东化工》2025年第3期125-128,共4页Guangdong Chemical Industry

基　　金：广州市教育局高校科研项目(202235420,202032873,202032865);广东省普通高校特色创新类项目(2022KTSCX306);广东省普通高校青年创新人才项目(2020KQNCX201);广东省中医药局科研项目(20231228)。

摘　　要：目的:研究金钗石斛和玫瑰石斛茎的低极性成分及抗肿瘤活性。方法:经95%乙醇提取,石油醚萃取,对两种石斛属植物茎中低极性成分进行GC-MS分析。以MTS法检测肿瘤细胞毒活性,筛选其乙醇提取物、石油醚萃取物的抗肿瘤活性。结果:金钗石斛茎低极性成分GC-MS检出匹配度大于85%的化合物有16种,相对含量占94.06%。其茎石油醚萃取物浓度在200μg/mL对白血病(HL-60)和结肠癌(SW480)两种肿瘤细胞抑制率分别为89.02%和70.73%。玫瑰石斛茎低极性成分GC-MS检出匹配度大于85%的化合物有3种,相对含量占88.87%。其茎乙醇萃取物浓度在200μg/mL对白血病(HL-60)、乳腺癌(MDA-MB-231)、结肠癌(SW480)3种肿瘤细胞抑制率分别为92.47%、89.67%和91.10%。结论:通过两种植物茎低极性成分的GC-MS分析及抗肿瘤活性研究,丰富了其化学成分的研究,进一步验证了其抗肿瘤活性。Objective:To study the low polarity components in the stem of Dendrobium nobile and Dendrobium crepidatum,as well as the anti-tumor activity of its’ethanol extracts and petroleum ether extracts.Methods:The stem of D.nobile and D.crepidatum were extracted with ethanol and were identified by GC-MS respectively.The relative contents of the chemical constituents were determined by area normalization method.Detect the cytotoxicity of tumor cells using MTS method and screen the anti-tumor activity of its ethanol extract and petroleum ether extract.Results:16 compounds with a matching degree greater than 85%detected by GC-MS for the low polarity components in the stem of D.nobile with a relative content of 94.06%.The inhibitory rates of its stem petroleum ether extract at a concentration of 200μg/mL on leukemia(HL-60)and colon cancer(SW480)tumor cells were 89.02%and 70.73%,respectively.32 compounds with a matching degree greater than 85%detected by GC-MS for the low polarity components in the stem of D.crepidatum with a relative content of 88.87%.The inhibitory rates of its stem petroleum ether extract at a concentration of 200μg/mL leukemia(HL-60),breast cancer(MDA-MB-231)and colon cancer(SW480)was 92.47%,89.67%and 91.10%,respectively.Conclusion:Through GC-MS analysis and anti-tumor activity research on the low polarity components of these two plants,the research on its chemical components has been enriched,and its anti-tumor activity has been further verified.

关 键 词：玫瑰石斛 金钗石斛 茎 低极性成分 GC-MS 抗肿瘤活性 

分 类 号：TQ[化学工程]